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Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH
4)
2SO
4, 50 mmol/L; KCl, 100 mmol/L; MgCl
2, 20 mmol/L; pH approximately 8.3 at +25°C
Unspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 μL after 1 hour incubation at +37°C.
Function test in PCR (50 pg human genomic DNA, 365 bp tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.",
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