Others Expand High Fidelity PCR System Expand High Fidelity PCR System For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only acc. to Art. 56 (3) and 3 no. 23 REACH Regulation. 3.6.17.2.5.1 Polymerase and buffer system for the generation of DNA fragments ranging from 3 kb to 27 kb 03 310 256 103 3 310 256 103 03310256103 3310256103 03310256103 Expand High Fidelity Expand High Fidelity PCR System Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as "Expand High Fidelity". Unit of measure is "kU". The enzyme is supplied without reaction buffer. 9012-90-2 en Proofreading blend for accurate amplification of genomic DNA targets up to 5 kb using PCR. en Use Expand High Fidelity PCR System for: Routine amplification of DNA fragments up to 5 kb from all DNAAmplification of DNA fragments up to 10 kb.Labeling of PCR products with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)Manufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control), including validation en Improve fidelity of PCR. Use this enzyme blend with its threefold greater accuracy than Taq Polymerase for more precise amplification of longer DNA templates. Maximize target yield. Minimize amplification of prematurely terminated products using an ideally formulated proofreading enzyme for increased full-length yields. en Enzyme blend consisting of Taq DNA Polymerase and Tgo DNA Polymerase. en Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCI, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C Volume activity: ≥3.5 U/μL RNases (MS2 RNA): Not detectable in up to 30 U after 1 hour incubation at +37°C. Function test in PCR (200 ng human genomic DNA, 4.8 kb tPA fragment): Corresponds to specification Stability: At -15 to -25°C within specification range for 24 months. en

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