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The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.6.6.4.59.1", "BrandName": "Expand High Fidelity PCR Buffer", "ProductNameAddition": "10x conc., with MgCl2", "ReferenceType": "Reagent", "Classification": [ { "IdentifierofStructureSystem": "OWP_Family", "NameofStructureSystem": "Product Families", "StructureNodeID": "570", "StructureGroupPath": "Expand", "StructureGroupName": "Expand" }, { "IdentifierofStructureSystem": "OWP_Product_Types", "NameofStructureSystem": "Product Types", "StructureNodeID": "70-009-00", "StructureGroupPath": "Raw Materials->Buffers", "StructureGroupName": "Buffers" }, { "IdentifierofStructureSystem": "Product_Grouping", "NameofStructureSystem": "Product Grouping", "StructureNodeID": "02-0136", "StructureGroupPath": "CustomBiotech->Molecular Raw Materials", "StructureGroupName": "Molecular Raw Materials" }, { "IdentifierofStructureSystem": "OWP_Organization", "NameofStructureSystem": "Organization", "StructureNodeID": "01-01-01-07-00", "StructureGroupPath": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureGroupName": "CustomBiotech and LifeScience" }, { "IdentifierofStructureSystem": "OWP_Techniques", "NameofStructureSystem": "Techniques", "StructureNodeID": "210-00", "StructureGroupPath": "PCR/qPCR", "StructureGroupName": "PCR/qPCR" }, { "IdentifierofStructureSystem": "Product_Solutions", "NameofStructureSystem": "Product Solutions", "StructureNodeID": "520", "StructureGroupPath": "CustomBiotech", "StructureGroupName": "CustomBiotech" }, { "IdentifierofStructureSystem": "Lab_Type", "NameofStructureSystem": "Lab Types", "StructureNodeID": "180-00", "StructureGroupPath": "Manufacturing", "StructureGroupName": "Manufacturing" }, { "IdentifierofStructureSystem": "Applications", "NameofStructureSystem": "Applications", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Disease_Areas", "NameofStructureSystem": "Disease Areas", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Pathogens", "NameofStructureSystem": "Pathogens", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" }, { "IdentifierofStructureSystem": "Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"Expand High Fidelity\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Proofreading blend for accurate amplification of genomic DNA targets up to 5 kb using PCR.", "Name": "Positioning" }, { "Language": "en", "Value": "Use Expand High Fidelity PCR System for:
  • Routine amplification of DNA fragments up to 5 kb from all DNA
  • Amplification of DNA fragments up to 10 kb.
  • Labeling of PCR products with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
  • Manufacture of amplification mixtures for regulated applications (e.g., in vitro diagnostics, quality control), including validation
", "Name": "Applications" }, { "Language": "en", "Value": "
  • Improve fidelity of PCR.
    Use this enzyme blend with its threefold greater accuracy than Taq Polymerase for more precise amplification of longer DNA templates.
  • Maximize target yield.
    Minimize amplification of prematurely terminated products using an ideally formulated proofreading enzyme for increased full-length yields.
", "Name": "Benefits" }, { "Language": "en", "Value": "Enzyme blend consisting of Taq DNA Polymerase and Tgo DNA Polymerase.", "Name": "Product Description" }, { "Language": "en", "Value": "Enzymes in Expand High Fidelity PCR System were originally isolated from the thermophilic eubacteria Thermus aquaticus (Taq) BM or Thermococcus gorgonarius (Tgo), both expressed in E. coli.
CAS Number: 9012-90-2
Enzyme acivities:
Taq Polymerase: Highly processive 5'-3' DNA polymerase; double-strand-specific 5'-3' exonuclease; no 3'-5' exonuclease activity
Tgo Polymerase: Highly processive 5'-3' DNA polymerase; double-strand-specific 3'-5' exonuclease (also known as proofreading activity); no 5'-3' exonuclease activity.
pH optimum: Approximately 8.9 (+20°C)
Temperature optimum:
Fragment length <3 kb: Approximately +72°C
Fragment length >3 kb: Approximately +68°C
Substrates: Incorporates dNTP, dUPT, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)
Recommended usage per 50 μL reaction: 2.5 U (0.7 μL)", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCI, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C
Volume activity: ≥3.5 U/μL
RNases (MS2 RNA): Not detectable in up to 30 U after 1 hour incubation at +37°C.
Function test in PCR (200 ng human genomic DNA, 4.8 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" } ] } } ] }

Expand High Fidelity PCR System

Polymerase and buffer system for the generation of DNA fragments ranging from 3 kb to 27 kb

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