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Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 220 mmol/L; MgCl2, 15 mmol/L; pH approximately 8.9 at +25°C
Unspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Function test in PCR (human genomic DNA, 4.8 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" } ] } } ] }

Expand High Fidelity PCR Buffer

10x conc., with MgCl2

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