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Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Exp.HF Buffer 10x w MgCl2 MPB\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use this buffer together with Expand High Fidelity PCR System.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"Exp.HF Buffer 10x w MgCl2 MPB\". Unit of measure is \"piece\".", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Standard reaction buffer for PCR using the Expand High Fidelity PCR System.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 220 mmol/L; MgCl2, 15 mmol/L; pH approximately 8.9 at +25°C
Unspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Function test in PCR (human genomic DNA, 4.8 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }

Expand High Fidelity PCR Buffer

10x conc., with MgCl2

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