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""
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"MaterialNum": "05917131103",
"MaterialDescription": "Expand HF Buffer 10x with MgCl2",
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"Language": "en",
"Value": "Use this buffer together with Expand High Fidelity PCR System.",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
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{
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"Value": "Will be supplied as \"Exp.HF Buffer 10x w MgCl2 MPB\". Unit of measure is \"piece\".",
"Country": "XG",
"Code": "CB - Order Information",
"Name": "CB - Order Information"
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"Country": "XG",
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Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH
4)
2SO
4, 220 mmol/L; MgCl
2, 15 mmol/L; pH approximately 8.9 at +25°C
Unspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Function test in PCR (human genomic DNA, 4.8 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.",
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