{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "EvoScript RNA Master", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "EvoScript RNA Master", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.17.2.1.120", "ProductNameAddition": "5x concentrated", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Store the master at −15 to −25°C.", "Name": "Storage Conditions (Product)" }, { "Language": "English", "Value": "Each lot of EvoScript RNA Master is tested to meet specifications of the RT-qPCR using a RT-qPCR assay on the LightCycler^® 480 Instrument II*.", "Name": "Quality Control" }, { "Language": "English", "Value": "Always run a negative control with the samples. To prepare a negative control, replace the template RNA with water. Contamination problems can be identified using negative control reactions.", "Name": "Control Reactions" }, { "Language": "English", "Value": "Use EvoScript RNA Master for hot start one-step RT-qPCR reactions that require high specificity and performance. It is compatible with hydrolysis probe and hybridization probe formats.", "Name": "Applications" }, { "Language": "English", "Value": "Appearance: Colorless solution
Function test RT-qPCR, G6PDH (on LC480 II with human reference RNA and G6PDH assay): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Suitable concentrations of hydrolysis probes range from 0.05 to 0.5 μM (final concentration in PCR). The recommended starting concentration is 0.1 μM each.", "Name": "Probe" }, { "Language": "English", "Value": "EvoScript RNA Master is a perfect master mix for convenient RT-qPCR reactions. It includes FastStart Taq DNA Polymerase and a designer polymerase for reverse transcription. It uses a sophisticated hot start system that includes chemical modification and aptamer-mediated hot start. This enables highly specific priming for both, reverse transcription and DNA amplification. The 1-vial composition is ideally suited for easy reaction assembly with just the addition of oligos and target RNA.", "Name": "Product Description" }, { "Language": "English", "Value": "Use any template RNA suitable for RT-qPCR in terms of purity, concentration, and absence of RT-PCR inhibitors.", "Name": "Sample Materials" }, { "Language": "English", "Value": "Suitable concentrations of PCR primers range from 0.2 to 0.5 μM (final concentration in RT-qPCR). The recommended starting concentration is 0.2 μM each.", "Name": "Primers" }, { "Language": "English", "Value": "EvoScript RNA Master (5x) is stable at room temperature for 24 hours and at a final (1x) dilution including primers and probes for 4 hours. The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).", "Name": "CB - Properties" }, { "Language": "English", "Value": "Will be supplied as \"EvoScript RNA Master, 5 mL\". Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "

• Save time.
  Just add primers, probes, and template to get started.
• Be flexible.
  Use a variety of sample materials (e.g., whole blood, FFPE).
• Achieve high sensitivity.
  Detect down to 10 copies of RNA target.
• Ready for automation.
  Set up your reaction at room temperature.
• Prevent carryover contamination.
  The mix is compatible with UNG protocols to prevent false positives.

", "Name": "Benefits" }, { "Language": "English", "Value": "True one-tube 5x hot start RT-PCR master mix for maximum ease-of-use. Ideal for high-specificity and high-precision one-step RT-qPCR reactions.", "Name": "Positioning" }, { "Language": "English", "Value": "Appearance: Colorless solution
Function test RT-qPCR, G6PDH (on LC480 II with human reference RNA and G6PDH assay): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.", "Name": "Specification" }, { "Language": "English", "Value": "EvoScript RNA Master (5x) is stable at room temperature for 24 hours and at a final (1x) dilution including primers and probes for 4 hours. The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Family", "NodeName": "Evoscript", "StructureSystemID": null, "NodeID": "160" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Reverse Transcriptases", "StructureSystemID": null, "NodeID": "70-047-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Techniques", "NodeName": "RT-PCR/qRT-PCR", "StructureSystemID": null, "NodeID": "300-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "07873468001", "MaterialDescription": "EvoScript RNA Master, 5 ml", "RegisteredProductName": "", "GTIN": "07613336131558", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "5 mL", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"EvoScript RNA Master, 5 mL\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }