{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "EvoScript RNA Master", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "EvoScript RNA Master", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "", "ProductNameAddition": "5x concentrated", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/Article_07873468001_im_en.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "Evoscript", "NodeID": "160" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Reverse Transcriptases", "NodeID": "70-047-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "RT-PCR/qRT-PCR", "NodeID": "300-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "07873468001", "MaterialDescription": "EvoScript RNA Master, 5 ml", "RegisteredProductName": "EvoScript RNA Master", "GTIN": "07613336131558", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "5 mL", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"EvoScript RNA Master, 5 mL\". Unit of measure is \"piece\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the master at −15 to −25°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Each lot of EvoScript RNA Master is tested to meet specifications of the RT-qPCR using a RT-qPCR assay on the LightCycler® 480 Instrument II*.", "Name": "Quality Control" }, { "Language": "en", "Value": "
Negative control
Always run a negative control with the samples. To prepare a negative control, replace the template RNA with water. Contamination problems can be identified using negative control reactions.", "Name": "Control Reactions" }, { "Language": "en", "Value": "Use EvoScript RNA Master for hot start one-step RT-qPCR reactions that require high specificity and performance. It is compatible with hydrolysis probe and hybridization probe formats.", "Name": "Applications" }, { "Language": "en", "Value": "Suitable concentrations of hydrolysis probes range from 0.05 to 0.5 μM (final concentration in PCR). The recommended starting concentration is 0.1 μM each.
For best results, design hydrolysis probes with a higher Tm than the Tm of the assay primers.
", "Name": "Probe" }, { "Language": "en", "Value": "EvoScript RNA Master is a perfect master mix for convenient RT-qPCR reactions. It includes FastStart Taq DNA Polymerase and a designer polymerase for reverse transcription. It uses a sophisticated hot start system that includes chemical modification and aptamer-mediated hot start. This enables highly specific priming for both, reverse transcription and DNA amplification. The 1-vial composition is ideally suited for easy reaction assembly with just the addition of oligos and target RNA.", "Name": "Product Description" }, { "Language": "en", "Value": "Use any template RNA suitable for RT-qPCR in terms of purity, concentration, and absence of RT-PCR inhibitors.", "Name": "Sample Materials" }, { "Language": "en", "Value": "Suitable concentrations of PCR primers range from 0.2 to 0.5 μM (final concentration in RT-qPCR). The recommended starting concentration is 0.2 μM each.", "Name": "Primers" }, { "Language": "en", "Value": "Will be supplied as \"EvoScript RNA Master, 5 mL\". Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
  • Save time.
    Just add primers, probes, and template to get started.
  • Be flexible.
    Use a variety of sample materials (e.g., whole blood, FFPE).
  • Achieve high sensitivity.
    Detect down to 10 copies of RNA target.
  • Ready for automation.
    Set up your reaction at room temperature.
  • Prevent carryover contamination.
    The mix is compatible with UNG protocols to prevent false positives.
", "Name": "Benefits" }, { "Language": "en", "Value": "True one-tube 5x hot start RT-PCR master mix for maximum ease-of-use. Ideal for high-specificity and high-precision one-step RT-qPCR reactions.", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Colorless solution
Function test RT-qPCR, G6PDH (on LC480 II with human reference RNA and G6PDH assay): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.", "Name": "Specification" }, { "Language": "en", "Value": "EvoScript RNA Master (5x) is stable at room temperature for 24 hours and at a final (1x) dilution including primers and probes for 4 hours. The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).", "Name": "Properties" } ] } } ] }

EvoScript RNA Master

5x concentrated




Technical documents

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