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Negative control
Always run a negative control with the samples. To prepare a negative control, replace the template RNA with water. Contamination problems can be identified using negative control reactions.", "Language": "en", "Country": "XG", "Code": "Control Reactions" }, { "Name": "Applications", "Value": "Use EvoScript RNA Master for hot start one-step RT-qPCR reactions that require high specificity and performance. It is compatible with hydrolysis probe and hybridization probe formats.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Probe", "Value": "Suitable concentrations of hydrolysis probes range from 0.05 to 0.5 μM (final concentration in PCR). The recommended starting concentration is 0.1 μM each.
For best results, design hydrolysis probes with a higher Tm than the Tm of the assay primers.
", "Language": "en", "Country": "XG", "Code": "Probe" }, { "Name": "Product Description", "Value": "EvoScript RNA Master is a perfect master mix for convenient RT-qPCR reactions. It includes FastStart Taq DNA Polymerase and a designer polymerase for reverse transcription. It uses a sophisticated hot start system that includes chemical modification and aptamer-mediated hot start. This enables highly specific priming for both, reverse transcription and DNA amplification. The 1-vial composition is ideally suited for easy reaction assembly with just the addition of oligos and target RNA.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Sample Materials", "Value": "Use any template RNA suitable for RT-qPCR in terms of purity, concentration, and absence of RT-PCR inhibitors.", "Language": "en", "Country": "XG", "Code": "Sample Materials" }, { "Name": "Primers", "Value": "Suitable concentrations of PCR primers range from 0.2 to 0.5 μM (final concentration in RT-qPCR). The recommended starting concentration is 0.2 μM each.", "Language": "en", "Country": "XG", "Code": "Primers" }, { "Name": "CB - Order Information", "Value": "Will be supplied as \"EvoScript RNA Master, 5 mL\". Unit of measure is \"piece\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Benefits", "Value": "
  • Save time.
    Just add primers, probes, and template to get started.
  • Be flexible.
    Use a variety of sample materials (e.g., whole blood, FFPE).
  • Achieve high sensitivity.
    Detect down to 10 copies of RNA target.
  • Ready for automation.
    Set up your reaction at room temperature.
  • Prevent carryover contamination.
    The mix is compatible with UNG protocols to prevent false positives.
", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Positioning", "Value": "True one-tube 5x hot start RT-PCR master mix for maximum ease-of-use. Ideal for high-specificity and high-precision one-step RT-qPCR reactions.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Specification", "Value": "Appearance: Colorless solution
Function test RT-qPCR, G6PDH (on LC480 II with human reference RNA and G6PDH assay): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "EvoScript RNA Master (5x) is stable at room temperature for 24 hours and at a final (1x) dilution including primers and probes for 4 hours. The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }

EvoScript RNA Master

5x concentrated


Ordering Information

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