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Always run a negative control with the samples. To prepare a negative control, replace the template RNA with water. Contamination problems can be identified using negative control reactions.",
"Name": "Control Reactions"
},
{
"Language": "en",
"Value": "Use EvoScript RNA Master for hot start one-step RT-qPCR reactions that require high specificity and performance. It is compatible with hydrolysis probe and hybridization probe formats.",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Suitable concentrations of hydrolysis probes range from 0.05 to 0.5 μM (final concentration in PCR). The recommended starting concentration is 0.1 μM each.
For best results, design hydrolysis probes with a higher Tm than the Tm of the assay primers.
",
"Name": "Probe"
},
{
"Language": "en",
"Value": "EvoScript RNA Master is a perfect master mix for convenient RT-qPCR reactions. It includes FastStart Taq DNA Polymerase and a designer polymerase for reverse transcription. It uses a sophisticated hot start system that includes chemical modification and aptamer-mediated hot start. This enables highly specific priming for both, reverse transcription and DNA amplification. The 1-vial composition is ideally suited for easy reaction assembly with just the addition of oligos and target RNA.",
"Name": "Product Description"
},
{
"Language": "en",
"Value": "Use any template RNA suitable for RT-qPCR in terms of purity, concentration, and absence of RT-PCR inhibitors.",
"Name": "Sample Materials"
},
{
"Language": "en",
"Value": "Suitable concentrations of PCR primers range from 0.2 to 0.5 μM (final concentration in RT-qPCR). The recommended starting concentration is 0.2 μM each.",
"Name": "Primers"
},
{
"Language": "en",
"Value": "Will be supplied as \"EvoScript RNA Master, 5 mL\". Unit of measure is \"piece\".",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "
Save time. Just add primers, probes, and template to get started.
Be flexible. Use a variety of sample materials (e.g., whole blood, FFPE).
Achieve high sensitivity. Detect down to 10 copies of RNA target.
Ready for automation. Set up your reaction at room temperature.
Prevent carryover contamination. The mix is compatible with UNG protocols to prevent false positives.
",
"Name": "Benefits"
},
{
"Language": "en",
"Value": "True one-tube 5x hot start RT-PCR master mix for maximum ease-of-use. Ideal for high-specificity and high-precision one-step RT-qPCR reactions.",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "Appearance: Colorless solution Function testRT-qPCR, G6PDH (on LC480 II with human reference RNA and G6PDH assay): Corresponds to reference Stability: At -15 to -25°C within specification range for 15 months.",
"Name": "Specification"
},
{
"Language": "en",
"Value": "EvoScript RNA Master (5x) is stable at room temperature for 24 hours and at a final (1x) dilution including primers and probes for 4 hours. The mix contains dUTP, so that it may be used with Uracil-DNA Glycosylase to prevent false positives arising from carryover contamination (i.e., contamination with amplified DNA).",
"Name": "Properties"
}
]
}
}
]
}
