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"Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator E Bio*
  • Control E Level 1 Bio*
  • Control E Level 2 Bio*
  • Control E Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Measuring Range
Ethanol concentration can be determined in following range:
  • ETOHB: 0.5 to 10.1 g/L (11 to 220 mmol/L)
  • ETHOD: 10 to 202 g/L (0.22 to 4.4 mol/L)

Conversion Factors
Conversion factors for formate concentration:
  • 1 mmol/L = 46.07 mg/L
  • 1 g/L = 21.71 mmol/L

Traceability
This method has been standardized against NIST-traceable standard materials.

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).

 Level 1Level 2Level 3
Mean50 mmol/L
(2.3 g/L)
100 mmol/L
(4.6 g/L)
150 mmol/L
(6.9 g/L)
CV in-run0.9 %1.1 %0.6 %
CV inter-run1.2 %1.1 %0.9 %
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
Test protocol
ETOHB is the standard protocol for ethanol determination. If the ethanol concentration of samples is expected to be higher than 220 mmol/L (10.1 g/L), then protocol ETOHD should be used (same protocol with pre-set 1:20 sample predilution). Alternatively, select another predilution factor (up to a factor of 1:20) in the analyzer software for testing samples with higher concentrations.
Cedex Bio HT test definition
Test definitionETOHB
(248)
ETOHD
(242)
Measuring range
     mmol/L
     g/L
Standard
11 – 220
0.5 – 10.1 
High
220 – 4,400
10 – 202
Control levelE 1, 2, 3Custom
Sample predilutionNo1:20
Measuring modeAbsorbanceAbsorbance
Abs. calc. modeKineticKinetic
Reaction modeR1-S-SRR1-S-SR
Reaction directionIncreaseIncrease
Wavelength A/B340/659 nm340/659 nm
Calc. first/last44/5444/54
Unitmmol/L, mg/Lmmol/L, mg/L
Pipetting parameters
Pipetting parametersETOHB
(248)
ETOHD
(242)
Water Diluent (Sample Predilution)No1:20
R150 μL 50 μL 
Sample (S)4 μL + 16 μL water4 μL + 16 μL water
SR50 μL50 μL
Total Volume120 μL120 μL
Calibration
Calibration
CalibratorCalibrator E Bio (CAL E, 07-1166-7)
Calibration modeLinear regression
Calibration interval56 days, and if recalibration is required due to QC results.
Quality control
Protocol ETOHB
  • Control E Level 1 Bio (CONE1, 07-1501-8)
  • Control E Level 2 Bio (CONE2, 07-1502-6)
  • Control E Level 3 Bio (CONE3, 07-1503-4)
Protocol ETOHD
  • Custom control: For quality control of ETOHD, use custom controls with ethanol concentrations in the high range of 15 to 175 g/L. The Control E Levels 1-3 cover the lower range of protocol ETOHB and are not suitable for ETOHD.

Use the recommended control material for ETOHB. Other suitable control material can also be used.
Control intervals and acceptance limits should be adapted to each lab\u00adoratory's individual requirements. If values do not fall within the defined limits, corrective measures and recalibration are required.
Conversion factors
Conversion factors for ethanol concentration:
  • 1 mmol/L = 46.07 mg/L
  • 1 g/L = 21.71 mmol/L
Traceability
This method has been standardized against NIST-traceable standard materials.
Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).
 Level 1Level 2Level 3
Mean50 mmol/L (2.3 g/L)100 mmol/L (4.6 g/L)150 mmol/L (6.9 g/L)
CV in-run [%]0.91.10.6
CV inter-run [%]1.21.10.9
", "Name": "Protocols" }, { "Language": "en", "Value": "Sample Handling
Due to the high volatility of ethanol, vials containing samples and controls must be kept tightly closed until just before measuring. After opening the vials, start the ethanol test on the instrument within 5 min to avoid reduced ethanol concentrations due to evaporation.
If additional tests need to be run using the same sample, then the ethanol test should be started first. Order other tests after sampling for the ethanol test has finished.
If sample results are flagged because results are higher than the measuring range, use another freshly opened sample vial for a rerun with predilution (ETOHD). Do not use the rerun function on the same open sample.
Do not use disinfectants that contain alcohol in the environment of the samples.
", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Ethanol can be used as source of carbon and energy in microbial fermentation, or it can be generated as product expecially in yeast cultures. For monitoring in a culture process or for control of fermentation products, ethanol can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using alcohol dehydrogenase.
Recovery: ±10%", "Name": "Specification" }, { "Language": "en", "Value": "
VialContentComposition
ETOHBReagent cassette,
100 tests
Reagent 1 (R1): Buffer, preservatives.
Reagent 2 (R2): Nicotinamide adenine dinucleotide, ≥3 mmol/L; alcohol dehydrogenase, ≥37 U/mL; stabilizers; preservatives.
", "Name": "Content" } ] } } ] }

Ethanol Bio HT

Test kit for the Cedex Bio HT Analyzer

cedex-ethanol-bio-ht

Overview

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Technical documents

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