{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Endoproteinase Lys-C Sequencing Grade lyophilizate", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "Endoproteinase Lys-C, Sequencing Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.3.7.1.2", "ProductNameAddition": "from Lysobacter enzymogenes, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Use Endoproteinase Lys-C, Sequencing Grade, for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.", "Name": "Applications" }, { "Language": "English", "Value": "Molecular weight: 33 kD (reduced), 30 kD (nonreduced)
pH optimum: 8.5-8.8
Inhibitors: DFP, TLCK, aprotinin, and leupeptin", "Name": "CB - Properties" }, { "Language": "English", "Value": "Will be supplied as ''Endoprot. Lys-C, Sequ., MPB 5 UG''. Unit of measure is ''piece''.", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Appearance: White lyophilizate
Activity (+25°C, Chromozyme^® PL): ≥1 U/bottle
Specific activity: ≥200 U/mg protein
Purity (SDS PAGE, homogeneity Phast-system^® ; 8-25%): ≥90%
Specificity (HPLC, melittin, after 1 hour incubation): ≥90%
Unspecific cleavage products after 18 hours: ≤5%
Stability: At +2 to +8°C within specification range for 12 months. Store dry.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Endoproteinase Lys-C, Sequencing Grade, is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from Lysobacter enzymogenes and is supplied as a lyophilizate.", "Name": "Product Description" }, { "Language": "English", "Value": "Endoproteinase Lys-C, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC. Purity is checked by HPLC and SDS PAGE using silver staining.", "Name": "Quality Control" }, { "Language": "English", "Value": "Endoproteinase Lys-C, Sequencing Grade, can be used for specific cleavage of peptides.", "Name": "Positioning" }, { "Language": "English", "Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Asp-N, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.", "Name": "Background Information" }, { "Language": "English", "Value": "Appearance: White lyophilizate
Activity (+25°C, Chromozyme^® PL): ≥1 U/bottle
Specific activity: ≥200 U/mg protein
Purity (SDS PAGE, homogeneity Phast-system^® ; 8-25%): ≥90%
Specificity (HPLC, melittin, after 1 hour incubation): ≥90%
Unspecific cleavage products after 18 hours: ≤5%
Stability: At +2 to +8°C within specification range for 12 months. Store dry.", "Name": "Specification" }, { "Language": "English", "Value": "Molecular weight: 33 kD (reduced), 30 kD (nonreduced)
pH optimum: 8.5-8.8
Inhibitors: DFP, TLCK, aprotinin, and leupeptin", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Protease Portfolio", "StructureSystemID": null, "NodeID": "02-0112" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Endoproteases", "StructureSystemID": null, "NodeID": "70-020-00" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11058533103", "MaterialDescription": "Endoprot.Lys-C,Sequ.,MPB 5 UG", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "Minimum order size of 100 μg
(pack size 5 μg)", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Endoprot. Lys-C, Sequ., MPB 5 UG''. Unit of measure is ''piece''." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }