{ "ProductData": { "ID": "3.1.3.7.1.2", "ProductType": "Others", "BrandName": "Endoproteinase Lys-C, Sequencing Grade", "ProductNameAddition": "from Lysobacter enzymogenes, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Endoproteinase Lys-C Sequencing Grade lyophilizate" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Endoproteases", "NodeID": "70-020-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Cell and Gene Therapy", "NodeID": "02-0133" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Protease Portfolio", "NodeID": "02-0112" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Enzymes", "NodeID": "02-0014" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11058533103", "MaterialDescription": "Endoprot.Lys-C,Sequ.,MPB 5 UG", "RegisteredProductName": "Endoproteinase Lys-C, Sequencing Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "5 μg", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Endoprot. Lys-C, Sequ., MPB 5 UG''. Unit of measure is ''piece''. Minimum order quantity is 100 μg" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use Endoproteinase Lys-C, Sequencing Grade, for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as ''Endoprot. Lys-C, Sequ., MPB 5 UG''. Unit of measure is ''piece''. Minimum order quantity is 100 μg.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Endoproteinase Lys-C, Sequencing Grade, is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from Lysobacter enzymogenes and is supplied as a lyophilizate.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Endoproteinase Lys-C, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC. Purity is checked by HPLC and SDS PAGE using silver staining.", "Country": "XG", "Code": "Quality Control", "Name": "Quality Control" }, { "Language": "en", "Value": "Endoproteinase Lys-C, Sequencing Grade, can be used for specific cleavage of peptides.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Asp-N, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.", "Country": "XG", "Code": "Background Information", "Name": "Background Information" }, { "Language": "en", "Value": "Appearance: White lyophilizate
Activity (+25°C, Chromozyme^® PL): ≥1 U/bottle
Specific activity: ≥200 U/mg protein
Purity (SDS PAGE, homogeneity Phast-system^® ; 8-25%): ≥90%
Specificity (HPLC, melittin, after 1 hour incubation): ≥90%
Unspecific cleavage products after 18 hours: ≤5%
Stability: At +2 to +8°C within specification range for 12 months. Store dry.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number:123175-82-6
Molecular weight: 33 kD (reduced), 30 kD (nonreduced)
pH optimum: 8.5-8.8
Inhibitors: DFP, TLCK, aprotinin, and leupeptin", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }