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{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Endoproteinase Lys-C Sequencing Grade lyophilizate", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Endoproteinase Lys-C, Sequencing Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.3.7.1.2", "ProductNameAddition": "from
Lysobacter enzymogenes
, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Solutions", "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Protease Portfolio", "NodeID": "02-0112" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials->Endoproteases", "NodeID": "70-020-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11058533103", "MaterialDescription": "Endoprot.Lys-C,Sequ.,MPB 5 UG", "RegisteredProductName": "Endoproteinase Lys-C, Sequencing Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "5 μg", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your
local representative
.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Endoprot. Lys-C, Sequ., MPB 5 UG''. Unit of measure is ''piece''. Minimum order quantity is 100 μg" } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use Endoproteinase Lys-C, Sequencing Grade, for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as ''Endoprot. Lys-C, Sequ., MPB 5 UG''. Unit of measure is ''piece''. Minimum order quantity is 100 μg.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Endoproteinase Lys-C, Sequencing Grade, is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from
Lysobacter enzymogenes
and is supplied as a lyophilizate.", "Name": "Product Description" }, { "Language": "en", "Value": "Endoproteinase Lys-C, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC. Purity is checked by HPLC and SDS PAGE using silver staining.", "Name": "Quality Control" }, { "Language": "en", "Value": "Endoproteinase Lys-C, Sequencing Grade, can be used for specific cleavage of peptides.", "Name": "Positioning" }, { "Language": "en", "Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Asp-N, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.", "Name": "Background Information" }, { "Language": "en", "Value": "
Appearance
: White lyophilizate
Activity
(+25°C, Chromozyme
®
PL): ≥1 U/bottle
Specific activity
: ≥200 U/mg protein
Purity
(SDS PAGE, homogeneity Phast-system
®
; 8-25%): ≥90%
Specificity
(HPLC, melittin, after 1 hour incubation): ≥90%
Unspecific cleavage
products after 18 hours: ≤5%
Stability
: At +2 to +8°C within specification range for 12 months. Store dry.", "Name": "Specification" }, { "Language": "en", "Value": "
CAS Number
:123175-82-6
Molecular weight
: 33 kD (reduced), 30 kD (nonreduced)
pH optimum
: 8.5-8.8
Inhibitors
: DFP, TLCK, aprotinin, and leupeptin", "Name": "Properties" } ] } } ] }
Endoproteinase Lys-C, Sequencing Grade
from
Lysobacter enzymogenes
, lyophilizate
Product Overview
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Trypsin, recombinant
Expressed in
Pichia pastoris
expressed in
Pichia pastoris
Papain
from
Carica papaya
, suspension
from
Carica papaya
, suspension
Pronase
from
Streptomyces griseus
, lyophilizate
from
Streptomyces griseus
, lyophilizate
Endoproteinase Glu-C
from
Staphylococcus aureus
V8, lyophilizate, salt-free
from
Staphylococcus aureus
V8, lyophilizate, salt-free
Endoproteinase LysC, rec. mutant
from
Pichia pastoris
from
Pichia pastoris
Endoproteinase Asp-N, Sequencing Grade
from a mutant of
Pseudomonas fragi
, lyophilizate
from a mutant of
Pseudomonas fragi
, lyophilizate
Collagenase I
liquid, mammalian free
liquid, mammalian free
Collagenase I/II
lyophilizate, mammalian free
lyophilizate, mammalian free
Collagenase II
liquid, mammalian free
liquid, mammalian free
Chromozym TRY
powder
powder
Collagenase P
from
Clostridium histolyticum
from
Clostridium histolyticum
Carboxypeptidase B, recombinant
from rat pancreas, expressed in
Pichia pastoris
, solution
from rat pancreas, expressed in
Pichia pastoris
, solution
Amino Acid Arylamidase
from Hog Kidney
from hog kidney
Residual Protein Trypsin Kit
Kit for determination of trypsin
Kit for determination of trypsin
Residual Protein Liberase Kit
ELISA kit for determination of Liberase
ELISA kit for determination of Liberase
Proteinase K, recombinant, PCR Grade
from
Titrirachium album
, expressed in
Pichia pastoris
, solution
from
Titrirachium album
, expressed in
Pichia pastoris
, solution
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