{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Endoproteinase Asp-N Sequencing Grade lyophilyzate", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Endoproteinase Asp-N, Sequencing Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.3.7.3.1", "ProductNameAddition": "from a mutant of Pseudomonas fragi, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Protease Portfolio", "NodeID": "02-0112" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Endoproteases", "NodeID": "70-020-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11058541103", "MaterialDescription": "Endoproteinase Asp-N Sequencing Grade", "RegisteredProductName": "Endoproteinase Asp-N, Sequencing Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": " 2 μg", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Endoproteinase Asp-N can be used for specific cleavage of peptides.", "Name": "Positioning" }, { "Language": "en", "Value": "Use Endoproteinase Asp-N, Sequencing Grade, for protein structure analysis and sequence analysis.", "Name": "Applications" }, { "Language": "en", "Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Lys-C, Sequencing Grade, Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.", "Name": "Background Information" }, { "Language": "en", "Value": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Endoproteinase Asp-N, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC.", "Name": "Quality Control" }, { "Language": "en", "Value": "
  • Obtain consistent and clear peptide sequencing results.
  • Minimize the risk of unknown peptide impurities by using this highly purified quality.
", "Name": "Benefits" }, { "Language": "en", "Value": "Endoproteinase Asp-N is a widely used metalloprotease, that specifically hydrolyzes peptide bonds at the amino side of aspargine and cysteine.
If cysteine is reduced or alkylated only -↓-Asp-X is cleaved. Endoproteinase Asp-N is isolated from Pseudomonas fragi mutant. The protease is supplied as lyophilizate.", "Name": "Product Description" }, { "Language": "en", "Value": "Appearance: White lyophilized substance
Activity (+37°C, with azocoll): ≥40 U/bottle
Specific activity: ≥20,000 U/mg protein
SDS PAGE (homogeneity; phastSystem®, 8-25%): ≥90%
Specificity (HPLC, with glucagon, cleavage after 1 h): ≥90%
Unspecific cleavage products (HPLC, melittin, after 4 hours incubation): ≤10%
Stability: At +2 to +8°C within specification range for 12 months; stored dry.", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number: 55576-49-3
Molecular weight: 27 kD
pH optimum: 7.0-8.0
Inhibitors: EDTA and α-phenanthroline", "Name": "Properties" } ] } } ] }

Endoproteinase Asp-N, Sequencing Grade

from a mutant of Pseudomonas fragi, lyophilizate

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