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"ProductType": "Others",
"BrandName": "Endoproteinase Asp-N, Sequencing Grade",
"ProductNameAddition": "from a mutant of
Pseudomonas fragi, lyophilizate",
"RegulatoryDisclaimer1": "For further processing only.",
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"RegulatoryDisclaimer2": null,
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"RegulatoryDisclaimer3": null,
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""
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""
],
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""
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"ProductNameGlobal": "Endoproteinase Asp-N Sequencing Grade lyophilyzate"
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"MaterialDescription": "Endoproteinase Asp-N Sequencing Grade",
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"PackSizePIM360": " 2 μg",
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"ProductSpec": [
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"ProductSpecVariant": {
"Chapters": [
{
"Name": "Positioning",
"Value": "Endoproteinase Asp-N can be used for specific cleavage of peptides.",
"Language": "en",
"Country": "XG",
"Code": "Positioning"
},
{
"Name": "Applications",
"Value": "Use Endoproteinase Asp-N, Sequencing Grade, for protein structure analysis and sequence analysis.",
"Language": "en",
"Country": "XG",
"Code": "Applications"
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{
"Name": "Background Information",
"Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Lys-C, Sequencing Grade, Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.",
"Language": "en",
"Country": "XG",
"Code": "Background Information"
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"Value": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces.",
"Language": "en",
"Country": "XG",
"Code": "CB - Order Information"
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{
"Name": "Quality Control",
"Value": "Endoproteinase Asp-N, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC.",
"Language": "en",
"Country": "XG",
"Code": "Quality Control"
},
{
"Name": "Benefits",
"Value": "
- Obtain consistent and clear peptide sequencing results.
- Minimize the risk of unknown peptide impurities by using this highly purified quality.
",
"Language": "en",
"Country": "XG",
"Code": "Benefits"
},
{
"Name": "Product Description",
"Value": "Endoproteinase Asp-N is a widely used metalloprotease, that specifically hydrolyzes peptide bonds at the amino side of aspargine and cysteine.
If cysteine is reduced or alkylated only -↓-Asp-X is cleaved. Endoproteinase Asp-N is isolated from
Pseudomonas fragi mutant. The protease is supplied as lyophilizate.",
"Language": "en",
"Country": "XG",
"Code": "Product Description"
},
{
"Name": "Specification",
"Value": "
Appearance: White lyophilized substance
Activity (+37°C, with azocoll): ≥40 U/bottle
Specific activity: ≥20,000 U/mg protein
SDS PAGE (homogeneity; phastSystem
®, 8-25%): ≥90%
Specificity (HPLC, with glucagon, cleavage after 1 h): ≥90%
Unspecific cleavage products (HPLC, melittin, after 4 hours incubation): ≤10%
Stability: At +2 to +8°C within specification range for 12 months; stored dry.",
"Language": "en",
"Country": "XG",
"Code": "Specification"
},
{
"Name": "Properties",
"Value": "
CAS Number: 55576-49-3
Molecular weight: 27 kD
pH optimum: 7.0-8.0
Inhibitors: EDTA and α-phenanthroline",
"Language": "en",
"Country": "XG",
"Code": "Properties"
}
]
}
}
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}