{ "ProductData": { "ID": "3.1.3.7.3.1", "ProductType": "Others", "BrandName": "Endoproteinase Asp-N, Sequencing Grade", "ProductNameAddition": "from a mutant of Pseudomonas fragi, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "OWP-approved", "RegulatoryDisclaimer2": "", "DisclaimerGroup2": null, "RegulatoryDisclaimer3": "", "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Endoproteinase Asp-N Sequencing Grade lyophilyzate" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Protease Portfolio", "NodeID": "02-0112" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Endoproteases", "NodeID": "70-020-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11058541103", "MaterialDescription": "Endoproteinase Asp-N Sequencing Grade", "RegisteredProductName": "Endoproteinase Asp-N, Sequencing Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": " 2 μg", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Endoproteinase Asp-N can be used for specific cleavage of peptides.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Use Endoproteinase Asp-N, Sequencing Grade, for protein structure analysis and sequence analysis.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Lys-C, Sequencing Grade, Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.", "Country": "XG", "Code": "Background Information", "Name": "Background Information" }, { "Language": "en", "Value": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Endoproteinase Asp-N, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC.", "Country": "XG", "Code": "Quality Control", "Name": "Quality Control" }, { "Language": "en", "Value": "
  • Obtain consistent and clear peptide sequencing results.
  • Minimize the risk of unknown peptide impurities by using this highly purified quality.
", "Country": "XG", "Code": "Benefits", "Name": "Benefits" }, { "Language": "en", "Value": "Endoproteinase Asp-N is a widely used metalloprotease, that specifically hydrolyzes peptide bonds at the amino side of aspargine and cysteine.
If cysteine is reduced or alkylated only -↓-Asp-X is cleaved. Endoproteinase Asp-N is isolated from Pseudomonas fragi mutant. The protease is supplied as lyophilizate.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Appearance: White lyophilized substance
Activity (+37°C, with azocoll): ≥40 U/bottle
Specific activity: ≥20,000 U/mg protein
SDS PAGE (homogeneity; phastSystem®, 8-25%): ≥90%
Specificity (HPLC, with glucagon, cleavage after 1 h): ≥90%
Unspecific cleavage products (HPLC, melittin, after 4 hours incubation): ≤10%
Stability: At +2 to +8°C within specification range for 12 months; stored dry.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number: 55576-49-3
Molecular weight: 27 kD
pH optimum: 7.0-8.0
Inhibitors: EDTA and α-phenanthroline", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }

Endoproteinase Asp-N, Sequencing Grade

from a mutant of Pseudomonas fragi, lyophilizate

logo

Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
error errorMessage
Sorry, we couldn't find the content you are looking for
Please try again later