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Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Positioning", "Value": "Endoproteinase Asp-N can be used for specific cleavage of peptides.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Applications", "Value": "Use Endoproteinase Asp-N, Sequencing Grade, for protein structure analysis and sequence analysis.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Background Information", "Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Lys-C, Sequencing Grade, Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "CB - Order Information", "Value": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Quality Control", "Value": "Endoproteinase Asp-N, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC.", "Language": "en", "Country": "XG", "Code": "Quality Control" }, { "Name": "Benefits", "Value": "
  • Obtain consistent and clear peptide sequencing results.
  • Minimize the risk of unknown peptide impurities by using this highly purified quality.
", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Product Description", "Value": "Endoproteinase Asp-N is a widely used metalloprotease, that specifically hydrolyzes peptide bonds at the amino side of aspargine and cysteine.
If cysteine is reduced or alkylated only -↓-Asp-X is cleaved. Endoproteinase Asp-N is isolated from Pseudomonas fragi mutant. The protease is supplied as lyophilizate.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Specification", "Value": "Appearance: White lyophilized substance
Activity (+37°C, with azocoll): ≥40 U/bottle
Specific activity: ≥20,000 U/mg protein
SDS PAGE (homogeneity; phastSystem®, 8-25%): ≥90%
Specificity (HPLC, with glucagon, cleavage after 1 h): ≥90%
Unspecific cleavage products (HPLC, melittin, after 4 hours incubation): ≤10%
Stability: At +2 to +8°C within specification range for 12 months; stored dry.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "CAS Number: 55576-49-3
Molecular weight: 27 kD
pH optimum: 7.0-8.0
Inhibitors: EDTA and α-phenanthroline", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }

Endoproteinase Asp-N, Sequencing Grade

from a mutant of Pseudomonas fragi, lyophilizate


Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
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