{ "ProductData": { "ID": "3.1.3.7.3.1", "ProductType": "Others", "BrandName": "Endoproteinase Asp-N, Sequencing Grade", "ProductNameAddition": "from a mutant of Pseudomonas fragi, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Endoproteinase Asp-N Sequencing Grade lyophilyzate" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-020-00", "StructureNodeStatus": "valid", "NodeName": "Endoproteases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0133", "StructureNodeStatus": "valid", "NodeName": "Cell and Gene Therapy" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0112", "StructureNodeStatus": "valid", "NodeName": "Protease Portfolio" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11058541103", "MaterialDescription": "Endoproteinase Asp-N Sequencing Grade", "RegisteredProductName": "Endoproteinase Asp-N, Sequencing Grade", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": " 2 μg", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Positioning", "Value": "Endoproteinase Asp-N can be used for specific cleavage of peptides.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Applications", "Value": "Use Endoproteinase Asp-N, Sequencing Grade, for protein structure analysis and sequence analysis.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Background Information", "Value": "Roche offers a broad portfolio of highly purified endoproteinases suitable for peptide sequencing or other specific application. Related products include Endoproteinase Lys-C, Sequencing Grade, Endoproteinase Glu-C, Sequencing Grade, Endoproteinase Arg-C, Sequencing Grade, and more.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "CB - Order Information", "Value": "Will be supplied as \"Endoproteinase Asp-N Sequencing Grade\". Unit of measure is \"piece\". Minimum order quantity is 30 pieces.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Quality Control", "Value": "Endoproteinase Asp-N, Sequencing Grade, is free of impurities, according to the current Quality Control procedures, that may interfere with separation of peptides in RP HPLC.", "Language": "en", "Country": "XG", "Code": "Quality Control" }, { "Name": "Benefits", "Value": "

• Obtain consistent and clear peptide sequencing results.
• Minimize the risk of unknown peptide impurities by using this highly purified quality.

", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Product Description", "Value": "Endoproteinase Asp-N is a widely used metalloprotease, that specifically hydrolyzes peptide bonds at the amino side of aspargine and cysteine.
If cysteine is reduced or alkylated only -↓-Asp-X is cleaved. Endoproteinase Asp-N is isolated from Pseudomonas fragi mutant. The protease is supplied as lyophilizate.", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Specification", "Value": "Appearance: White lyophilized substance
Activity (+37°C, with azocoll): ≥40 U/bottle
Specific activity: ≥20,000 U/mg protein
SDS PAGE (homogeneity; phastSystem^®, 8-25%): ≥90%
Specificity (HPLC, with glucagon, cleavage after 1 h): ≥90%
Unspecific cleavage products (HPLC, melittin, after 4 hours incubation): ≤10%
Stability: At +2 to +8°C within specification range for 12 months; stored dry.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "CAS Number: 55576-49-3
Molecular weight: 27 kD
pH optimum: 7.0-8.0
Inhibitors: EDTA and α-phenanthroline", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }