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"ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"DNase I rec RNase free in Glycerol\". Unit of measure is \"kU\".", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "DNase I is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides. It digests DNA templates thereby purifing (m)RNA during in vitro synthesis. In molecular diagnostics applications, DNase I can be used to prepare genomic DNA-free RNA templates for RT-PCR.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Use the enzyme to digest DNA impurities during the synthesis and purification of RNA. This GMP Grade product is suitable for in vitro mRNA synthesis in the production of vaccines and therapeutics. Its main features include:

• free of antibiotic
• manufactured under GMP
• supplied with a TSE/BSE certificate
• supplied with supplementary documents for regulatory submissions.

", "Country": "XG", "Code": "Benefits", "Name": "Benefits" }, { "Language": "en", "Value": "DNase I, rec., RNase-free, is an essential enzyme for efficient digestion of DNA during RNA purification.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "No animal-derived material is added in fermentation, purification and final formulation of this enzyme.", "Country": "XG", "Code": "Quality Control", "Name": "Quality Control" }, { "Language": "en", "Value": "EC Number: 3.1.21.1
Molecular weight: 39 kD
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA.", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Colorless to slightly yellowish, solution
Volume activity (calf thymus DNA): 9-14 kU/mL
Unit Definition: One unit according to Kunitz is the amount of enzyme required to produce an increase of 0.001/minute/mL extinction coefficient at 260 nm under test conditions.
Volume activity (calf thymus DNA, modified buffer system): No limit
Proteases (up to 50 U resorufin-marked casein after 17 h; 37°C): Not detectable
Ribonucleases (up to 10 U with MS II RNA/4 h / 37°C): Not detectable
Stability: At -15 to -25°C within specification range for 24 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }