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Unit of measure is \"kU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "DNase I is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides. It digests DNA templates thereby purifing (m)RNA during in vitro synthesis. In molecular diagnostics applications, DNase I can be used to prepare genomic DNA-free RNA templates for RT-PCR.", "Name": "Applications" }, { "Language": "en", "Value": "Use the enzyme to digest DNA impurities during the synthesis and purification of RNA. This GMP Grade product is suitable for in vitro mRNA synthesis in the production of vaccines and therapeutics. Its main features include:
  • free of antibiotic
  • manufactured under GMP
  • supplied with a TSE/BSE certificate
  • supplied with supplementary documents for regulatory submissions.
", "Name": "Benefits" }, { "Language": "en", "Value": "DNase I, rec., RNase-free, is an essential enzyme for efficient digestion of DNA during RNA purification.", "Name": "Positioning" }, { "Language": "en", "Value": "No animal-derived material is added in fermentation, purification and final formulation of this enzyme.", "Name": "Quality Control" }, { "Language": "en", "Value": "EC Number: 3.1.21.1
Molecular weight: 39 kD
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA.", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Colorless to slightly yellowish, solution
Volume activity (calf thymus DNA): 9-14 kU/mL
Unit Definition: One unit according to Kunitz is the amount of enzyme required to produce an increase of 0.001/minute/mL extinction coefficient at 260 nm under test conditions.
Volume activity (calf thymus DNA, modified buffer system): No limit
Proteases (up to 50 U resorufin-marked casein after 17 h; 37°C): Not detectable
Ribonucleases (up to 10 U with MS II RNA/4 h / 37°C): Not detectable
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" } ] } } ] }

DNase I, rec., RNase-free, GMP Grade

from bovine pancreas, expressed in Pichia pastoris, solution

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mrna

Reliable DNA digestion


DNase I is an essential enzyme for efficient digestion of DNA during RNA purification. For any sensitive RNA-based application, the quality of input RNA matters. Manufactured in GMP Grade quality and without the use of antibiotics, this Roche CustomBiotech DNase I is especially developed to meet the needs of the stringent mRNA therapeutics and vaccine manufacturers.

Achieve reliable results with pure, undegraded and stable RNA. Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high yield and quality of your RNA.

produced using validated manufacturing and cleaning processes up to the final Animal-Origin-Free process (AOF): The product is manufactured without using any animal, human or cell culture derived material from TSE-relevant animal species acc. to EMA/410/01 Rev. 3. A statement regarding TSE/BSE and viral safety is available.

during fermentation, no antibiotics are used. Therefore, allergic reactions to antibiotics is not a factor in your overall risk analysis.

We work with you to provide the necessary technical documents for your regulatory submissions.

Changing reagent quality between pre-clinical development and commercialization poses a risk. Secure your processes by using the best available quality from the start.

Reach out to your regional CustomBiotech representative for more information
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Overview

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Technical documents

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