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Unit of measure is ''piece\". Minimum order quantity is 20 pieces.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "DNase I is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides. It digests DNA templates thereby purifing (m)RNA during in vitro synthesis. In molecular diagnostics applications, DNase I can be used to prepare genomic DNA-free RNA templates for RT-PCR.", "Name": "Applications" }, { "Language": "en", "Value": "DNase I, rec., RNase-free, is an essential enzyme for efficient digestion of DNA during RNA purification.", "Name": "Positioning" }, { "Language": "en", "Value": "Manufactured under GMP (Good Manufacturing Practice) regulations.
No animal-derived material is added in fermentation, purification and final formulation of this enzyme.", "Name": "Quality Control" }, { "Language": "en", "Value": "Use the enzyme to digest DNA impurities during the synthesis and purification of RNA. This GMP Grade product is suitable for in vitro mRNA synthesis in the production of vaccines and therapeutics. Its main features include:
  • free of antibiotic
  • manufactured under GMP
  • supplied with a TSE/BSE certificate
  • supplied with supplementary documents for regulatory submissions.
", "Name": "Benefits" }, { "Language": "en", "Value": "EC Number: 3.1.21.1
Molecular weight: 39 kD
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA.", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: White powder
Activity (with calf thymus DNA): ≥4.0 kU/vial
Ribonucleases (up to 15 U with MS II RNA /4 h/ 37°C): Not detectable
Manufactured acc. to GMP: Corresponds to specification
Filtered through 0.2 μm pore size Membrane, microbiologically tested: Corresponds to specification
Stability: At +2 to +8°C within specification range for 12 months.", "Name": "Specification" } ] } } ] }

DNase I, rec., RNase-free, GMP Grade

from bovine pancreas, expressed in Pichia pastoris, lyophilizate

rna

The certainty of a clean start - reliable DNA digestion

From RT-PCR to transcriptome sequencing, sensitive and specific RNA-based applications build on high-quality RNA input, free of DNA. DNase I isolated from bovine pancreas is commonly used to hydrolyze and remove contaminating DNA. 

 

  • Count on reliable DNA digestion and removal
    Proven enzymatic activity efficiently clears samples of DNA for demanding downstream applications.

  • Maintain RNA integrity from the start
    Stringent purity criteria enable isolating full-length RNA that serves as a template even at the 3’ end. 

  • Boost manufacturing and product quality
    Narrow performance and purity specifications eliminate variability in processes and outcomes.

  • Meet high standards in industry
    Animal-free and optionally GMP-conform, our DNase I is easily integrated into standing processes.

Overview

Ordering

Technical documents

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