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It is a glycoprotein of a molecular weight of approximately 39 kD. DNase I, recombinant, Grade I, is a DNA-specific endonuclease that hydrolyzes phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
DNase I, recombinant, Grade I, is manufactured using state-of-the-art processes yielding animal component-free material.", "Country": "XG", "Code": "Product Description", "Name": "Product Description" }, { "Language": "en", "Value": "Recombinant DNase I is an essential tool for all applications requiring DNA-free RNA templates.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "DNase I, recombinant, Grade I, is suitable for:

• Isolation of DNA-free RNA produced by in vitro transcription
• Producing DNA-free preparations of protein and RNA:
  - To ensure that RT-PCR templates are free of genomic DNA
  - To remove DNA templates after in vitro transcription of RNA
• Nick-translation labeling of DNA with added DNA polymerase I
• Determining the \"footprint\" of a DNA-binding protein
• Microarray analysis

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05952077103: Will be supplied as \"DNase I recombinant 510 KU\". Unit of measure is \"piece\". Minimum order quantity is 1 piece.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "

• Achieve reliable results with undegraded and stable RNA.
  Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high sensitivity of your RT-PCR assay.
• Be compliant with regulatory requirements.
  DNase as recombinant enzyme is free of animal-derived materials.

", "Country": "XG", "Code": "Benefits", "Name": "Benefits" }, { "Language": "en", "Value": "Nomenclature: DNase I
CAS Number: 9003-98-9
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: White to slightly yellowish lyophilizate
Activity (calf thymus DNA, hydrous solution): ≥10 kU/vial lyophilizate (cat.no. 03724778103);
450.0-562.5 kU (cat.no. 0592077103)
Activity (calf thymus DNA, modified buffer system): No limit
Unit definition: One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1 mL at 260 nm.
Proteases (resorufin-marked casein): Not detectable in up to 50 U after 17 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 2 U after 4 hours incubation at +37°C.
Stability: At +2 to +8°C within specification range for 24 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }