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- To remove DNA templates after in vitro transcription of RNA - Nick-translation labeling of DNA with added DNA polymerase I
- Determining the \"footprint\" of a DNA-binding protein
- Microarray analysis
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Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high sensitivity of your RT-PCR assay. - Be compliant with regulatory requirements.
DNase as recombinant enzyme is free of animal-derived materials.
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Nomenclature: DNase I
CAS Number: 9003-98-9
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA",
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Appearance: White to slightly yellowish lyophilizate
Activity (calf thymus DNA, hydrous solution): ≥10 kU/vial lyophilizate (cat.no. 03724778103);
450.0-562.5 kU (cat.no. 0592077103)
Activity (calf thymus DNA, modified buffer system): No limit
Unit definition: One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1 mL at 260 nm.
Proteases (resorufin-marked casein): Not detectable in up to 50 U after 17 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 2 U after 4 hours incubation at +37°C.
Stability: At +2 to +8°C within specification range for 24 months.",
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