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It is a glycoprotein of a molecular weight of approximately 39 kD. DNase I, recombinant, Grade I, is a DNA-specific endonuclease that hydrolyzes phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
DNase I, recombinant, Grade I, is manufactured using state-of-the-art processes yielding animal component-free material.", "Name": "Product Description" }, { "Language": "en", "Value": "Recombinant DNase I is an essential tool for all applications requiring DNA-free RNA templates.", "Name": "Positioning" }, { "Language": "en", "Value": "DNase I, recombinant, Grade I, is suitable for:
  • Isolation of DNA-free RNA produced by in vitro transcription
  • Producing DNA-free preparations of protein and RNA:
    - To ensure that RT-PCR templates are free of genomic DNA
    - To remove DNA templates after in vitro transcription of RNA
  • Nick-translation labeling of DNA with added DNA polymerase I
  • Determining the \"footprint\" of a DNA-binding protein
  • Microarray analysis
", "Name": "Applications" }, { "Language": "en", "Value": "03724778103: Will be supplied as \"DNase I rec RGI (10 KU)\". Unit of measure is \"piece\". Minimum order quantity is 20 pieces.
05952077103: Will be supplied as \"DNase I recombinant 510 KU\". Unit of measure is \"piece\". Minimum order quantity is 1 piece.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "
  • Achieve reliable results with undegraded and stable RNA.
    Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high sensitivity of your RT-PCR assay.
  • Be compliant with regulatory requirements.
    DNase as recombinant enzyme is free of animal-derived materials.
", "Name": "Benefits" }, { "Language": "en", "Value": "Nomenclature: DNase I
CAS Number: 9003-98-9
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: White to slightly yellowish lyophilizate
Activity (calf thymus DNA, hydrous solution): ≥10 kU/vial lyophilizate (cat.no. 03724778103);
450.0-562.5 kU (cat.no. 0592077103)
Activity (calf thymus DNA, modified buffer system): No limit
Unit definition: One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1 mL at 260 nm.
Proteases (resorufin-marked casein): Not detectable in up to 50 U after 17 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 2 U after 4 hours incubation at +37°C.
Stability: At +2 to +8°C within specification range for 24 months.", "Name": "Specification" } ] } } ] }

DNase I, recombinant, Grade I

from bovine pancreas, expressed in Pichia pastoris, lyophilizate

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