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In mRNA in vitro synthesis applications, it helps purify the mRNA by digesting the DNA impurities in the reaction mix. In other molecular diagnostics applications, DNase I can be used to prepare genomic DNA-free RNA templates for RT-PCR.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Benefits", "Value": "Achieve reliable results with pure, undegraded and stable RNA. Rely on the highly purified and rigorously tested product that excludes RNase activity ensuring high yield and quality of your RNA.

• animal-origin-free (AOF)
• free of antibiotics
• extended testing for impurities
• manufactured under GMP
• supplied with supplementary documents for regulatory submissions.

", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Specification", "Value": "Appearance: Colorless to slightly yellowish, solution
Volume activity (calf thymus DNA): 12-16 kU/mL (Kunitz)
Identity: Confirmed
Unit Definition: One unit according to Kunitz is the amount of enzyme required to hydrolyze the calf thymus DNA at 25°C and pH 5.0 to produce an increase of 0.001/min extinction coefficient at 260 nm under the test conditions.
Protein (abs. 280 nm): ≥ 1.0 mg/mL
Purity (SDS-PAGE): ≥ 95 %
Proteases (up to 50 U resorufin-marked casein after 17 h; 37°C): Not detectable
Ribonucleases (up to 10 U with MS II RNA/4 h / 37°C): Not detectable
Endotoxins: < 5 EU/mL
Bioburden: < 5 CFU/mL
Host cell protein: ≤ 100 ppm
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -15 to -25°C within specification range for 18 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "CB - Order Information", "Value": "09852093103: Will be supplied as 'DNase I rec., GMP Grade AOF'. Unit of measure is \"mL\".
09873562001:  Will be supplied as 'DNase I rec., GMP Grade AOF. 200 kU', Unit of measure is \"PC\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" } ] } } ] }