{ "ProductData": { "ID": "3.1.1.4.8.2", "ProductType": "Others", "BrandName": "D-Lactate Dehydrogenase (D-LDH), Grade II", "ProductNameAddition": "from Lactobacillus delbrückii, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "D-Lactate Dehydrogenase D-LDH Grade II" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-014-00", "StructureNodeStatus": "valid", "NodeName": "Dehydrogenases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0011", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "06-00-00", "StructureNodeStatus": "valid", "NodeName": "Enzyme Testing" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10679666103", "MaterialDescription": "D(-)-Lactate Dehydrogenase (D-LDH)", "RegisteredProductName": "D-Lactate Dehydrogenase (D-LDH), Grade II", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''D(-)-Lactate Dehydrogenase (D-LDH)''. Unit of measure is ''MU''." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Applications", "Value": "Use D-Lactate Dehydrogenase (D-LDH), Grade II, in a variety of diagnostic tests, e.g., in the determination of alanine aminotransferases, lactate or pyruvate. Used for the removal of pyruvate in determinations working with NADH (i.e., triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Positioning", "Value": "Dehydrogenase that catalyzes the interconversion of D(-)-lactate to pyruvate.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "CB - Order Information", "Value": "Will be supplied as ''D(-)-Lactate Dehydrogenase (D-LDH)''. Unit of measure is ''MU''.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Properties", "Value": "Nomenclature: D-lactate:NAD⁺ oxidoreductase
CAS Number: 9028-36-8
Michaelis constants (Tris maleate buffer, pH 8.0, +25°C):
D-lactate: 0.7 x 10^-1 mol/L (NAD, 2 mmol/L)
Pyruvate: 1.2 x 10^-3 mol/L (NADH, 0.1 mmol/L)
NADH: 7.1 x 10^-5 mol/L (pyruvate, 20 mmol/L)
pH optimum: 7.0
pH stability: 4.0-10.0
Thermal stability: Up to +50°C
Specificity: Lactate dehydrogenase is specific for D(-)-lactate, L(+)-lactate does not react.", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: White to yellowish lyophilizate
Solubility: Clear, colorless to slightly yellowish solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 5.7-6.7
Activity (+25°C, pyruvate): ≥150 U/mg lyophilizate
Specific activity: ≥300 U/mg protein
Protein (Biuret): No limit, approximately 0.4-0.7 mg/mg lyophilizate
Contaminants (expressed as percentage of D-Lactate Dehydrogenase activity):
Alcohol dehydrogenase: ≤0.01
GIucose dehydrogenase: ≤0.01
Malate dehydrogenase: ≤0.1
Succinate dehydrogenase: ≤0.01
Stability: At +2 to +8°C within specification range for 12 months. Store dry.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }