{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "COT Human DNA CGH Grade", "DisclaimerGroup1": "Other - Non IVD (DG_02)", "ControlTissue": [ "" ], "BrandName": "COT Human DNA, CGH Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.2.2.14.2", "ProductNameAddition": "from human male placenta DNA, enriched for repetitive sequences, solution", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Obtain best results for in situ suppression (CISS) hybridization, DNA microarray application and many other hybridization applications. With the COT Human DNA, CGH Grade, reproducible and sensitive measurements of dsDNA concentration are possible.", "Name": "Positioning" }, { "Language": "English", "Value": "The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS) such as SINEs (small interspersed repetitive elements, e.g., Alu-elements) and LINEs (large interspersed repetitive elements, e.g., L1-elements) are distributed ubiquitously throughout the genome. COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.", "Name": "Product Description" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
COT Human DNA (A₂₆₀, water, 1 AB=50 μg/mL): ≥1.0 mg/mL
Fluorometrical determination of concentration: 1.0-1.5 mg/mL
Y-Chromosom (recovered exclusively from male human placenta): Corresponds to specification
A₂₆₀/A₂₈₀: 1.6-2.0
Absence of HIV 1/2 and HCV/HBV: Corresponds to specification
Performance test using gel electrophoretic separation (4% agarose gel without RE cleavage): middle chain length: 50-300 bp
Comparable intensity to previous lot: Corresponds to specification
Stability: At -15 to -25°C within specification range for 18 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Will be supplied as \"COT Human DNA, CGH Grade\". Unit of measure is \"mL\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Use the COT Human DNA in the following applications:

• Nucleic acid labeling and detection
• DNA microarray applications such as comparative genome hybridization and sequence capture
• Complex hybridization of human nucleic acids like FISH

In microarray applications, COT Human DNA is used in hybridization solutions to block repetitive DNA sample sequences from nonspecific hybridizations. In filter and other hybridization techniques, COT Human DNA is also used in prehybridization solutions to inactivate nonspecific target binding sites.", "Name": "Applications" }, { "Language": "English", "Value": "The product is HIV tested.", "Name": "Quality Control" }, { "Language": "English", "Value": "Repetitive elements (IRS) present in a probe (e.g., cosmids, YACs, chromosome painting probes) generate nonspecific hybridization signals that are distributed over the whole chromosome or genome. To enable specific hybridization of the probe to the chromosomal target site (e.g., single-copy sequences or low-copy repeats), the probe must be denatured in the presence of excess unlabeled COT Human DNA. This DNA serves as a competitor. In a subsequent preannealing step, the repetitive probe elements rapidly hybridize to excess repeats in the Cot Human DNA, while most of the specific probe sequences remain single stranded, and can thus hybridize to their chromosomal targets. This technique is known as chromosomal in situ suppression (CISS) hybridization.", "Name": "Background Information" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
COT Human DNA (A₂₆₀, water, 1 AB=50 μg/mL): ≥1.0 mg/mL
Fluorometrical determination of concentration: 1.0-1.5 mg/mL
Y-Chromosom (recovered exclusively from male human placenta): Corresponds to specification
A₂₆₀/A₂₈₀: 1.6-2.0
Absence of HIV 1/2 and HCV/HBV: Corresponds to specification
Performance test using gel electrophoretic separation (4% agarose gel without RE cleavage): middle chain length: 50-300 bp
Comparable intensity to previous lot: Corresponds to specification
Stability: At -15 to -25°C within specification range for 18 months.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Additional Applications", "StructureSystemID": null, "NodeID": "02-0073" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Nucleic Acids", "StructureSystemID": null, "NodeID": "70-038-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ ] }, "Product2Products": { "Product2ProductReference": [ ] } }