{ "ProductData": { "ID": "3.1.4.1.12.1", "ProductType": "Others", "BrandName": "Cholesterol Esterase, Grade II", "ProductNameAddition": "from Pseudomonas species, lyophilizate", "RegulatoryDisclaimer1": null, "DisclaimerGroup1": null, "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Cholesterol Esterase Grade II" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-022-00", "StructureNodeStatus": "valid", "NodeName": "Esterases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0011", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "11-00-00", "StructureNodeStatus": "valid", "NodeName": "Lipid Testing" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11015923103", "MaterialDescription": "CholesterolEsterase from Pseudom.species", "RegisteredProductName": "Cholesterol Esterase, Grade II", "GTIN": "14038377155491", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Cholesterol Esterase from Pseudom.species\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "CB - Order Information", "Value": "Will be supplied as \"Cholesterol Esterase from Pseudom.species\". Unit of measure is \"MU\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Applications", "Value": "Use Cholesterol Esterase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 10 634 522 103 or 11 479 709 103.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Positioning", "Value": "Hydrolase that splits fatty acids from sterols.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Specification", "Value": "Appearance: Slightly yellowish lyophilizate
Solubility: Clear, colorless or yellowish to brownish solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Ficoll (colorimetric): 40-80 mg/100 mg lyophilizate
Activity (+25°C, cholesterol oleate): ≥30 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Protein (Biuret): 0.1-0.4 mg/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤200 U/mg lyophilizate
Glycerokinase: ≤0.001
GOD: ≤0.001
Hexokinase: ≤0.005
\"NADH oxidase\": ≤0.005
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "Nomenclature: Sterol-ester acylhydrolase
CAS Number: 9026-00-0
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10^-5 mol/L
Inhibitors: Heavy metals such as Cu²⁺, Ag⁺, Zn²⁺
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure)pH optimum
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure)pH stability
Thermal stability: Below +20°C (see figure)Thermal stability
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }