{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Cholesterol Esterase Grade II", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "Cholesterol Esterase, Grade II", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.1.4.1.12.1", "ProductNameAddition": "from Pseudomonas species, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Esterases", "NodeID": "70-022-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Clinical Chemistry Raw Materials", "NodeID": "02-0011" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Lipid Testing", "NodeID": "11-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11015923103", "MaterialDescription": "CholesterolEsterase from Pseudom.species", "RegisteredProductName": "Cholesterol Esterase, Grade II", "GTIN": "14038377155491", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Cholesterol Esterase from Pseudom.species\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"Cholesterol Esterase from Pseudom.species\". Unit of measure is \"MU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use Cholesterol Esterase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 10 634 522 103 or 11 479 709 103.", "Name": "Applications" }, { "Language": "en", "Value": "Hydrolase that splits fatty acids from sterols.", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Slightly yellowish lyophilizate
Solubility: Clear, colorless or yellowish to brownish solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Ficoll (colorimetric): 40-80 mg/100 mg lyophilizate
Activity (+25°C, cholesterol oleate): ≥30 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Protein (Biuret): 0.1-0.4 mg/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤200 U/mg lyophilizate
Glycerokinase: ≤0.001
GOD: ≤0.001
Hexokinase: ≤0.005
\"NADH oxidase\": ≤0.005
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months.", "Name": "Specification" }, { "Language": "en", "Value": "Nomenclature: Sterol-ester acylhydrolase
CAS Number: 9026-00-0
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10-5 mol/L
Inhibitors: Heavy metals such as Cu2+, Ag+, Zn2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure)
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure)
Thermal stability: Below +20°C (see figure)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.", "Name": "Properties" } ] } } ] }

Cholesterol Esterase, Grade II

from Pseudomonas species, lyophilizate

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