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"Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Store the reagent cassette at +2 to +8°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Keep at +2 to +15°C for on-board use. After first use on the instrument, the reagents are stable for up to 6 weeks.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator C Bio*
  • Control C Level 1 Bio*
  • Control C Level 2 Bio*
  • Control C Level 3 Bio*
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "This product is intended for quantitative determination of calcium in aqueous solutions using the Cedex Bio HT Analyzer.", "Name": "Product Description" }, { "Language": "en", "Value": "
VialContentComposition
CA2BReagent cassette,
300 tests
Reagent 1 (R1): CAPSO, 557 mmol/L; pH 10.0; 5-nitro-5′-methyl-BAPTA (NM-BAPTA), 2 mmol/L; non-reactive surfactant; preservative.
Start Reagent (SR): EDTA, 7.5 mmol/L, pH 7.3; non-reactive surfactant; preservative.
", "Name": "Content" }, { "Language": "en", "Value": "Measuring Range
Calcium concentration can be determined in following range:
  • CA2B: 8 - 200 mg/L (0.2 - 5.0 mmol/L)
  • CA2D: 80 - 2000 mg/L (2 - 50 mmol/L),
up to 20 g/L (500 mmol/L) with automatic dilution

Conversion Factors
Conversion factors for phosphate concentration:
  • 1 mmol/L = 40.08 mg/L
  • 1 mg/L = 0.02495 mmol/L

Traceability
This method has been standardized against a primary standard material (SRM 956 c level 2).

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).
 Level 1Level 2Level 3
Mean0.59 mmol/L
(23.7 mg/L)\t   
2.53 mmol/L
(101 mg/L)\t   
4.48 mmol/L
(180 mg/L)
CV in-run2.9 %1.8 %1.8 %
CV inter-run   3.5 %1.9 %2.1 %
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
Test protocol
Cedex Bio HT test definition
Test definitionCA2B
(745)
CA2D
(790)
Measuring range
     mmol/L
     mg/L
Standard
0.2 – 5.0
8 – 200
High
2 – 50(1) 
80 – 2,000(1)
Control levelC 1, 2, 3C 3
Sample predilutionNoNo
Measuring modeAbsorbanceAbsorbance
Abs. calc. modeEndpointEndpoint
Reaction modeR1-S-SRR1-S-SR
Reaction directionDecreaseDecrease
Wavelength A/B340/378 nm340/378 nm
Calc. first/last33/3633/36
Unitmmol/L, mg/Lmmol/L, mg/L
  1. Up to 500 mmol/L (20 g/L) with automatic dilution.
Pipetting parameters
Pipetting parametersCA2B
(745)
CA2D
(790)
Water Diluent (Sample Predilution)NoNo
R120 μL + 130 μL water20 μL + 130 μL water
Sample (S)3 μL + 30 μL water3 μL + 30 μL water
SR20 μL20 μL
Total Volume203 μL203 μL
Calibration
Calibration
CalibratorCalibrator C Bio (CAL C, 07-1110-1)
Calibration modeLinear regression
Calibration intervalEach new lot, and if recalibration is required due to QC results.
Quality control
Protocol CA2B
  • Control C Level 1 Bio (CONC1, 07-1112-8)
  • Control C Level 2 Bio (CONC2, 07-1113-6)
  • Control C Level 3 Bio (CONC3, 07-1114-4)
Protocol CA2D
  • Control C Level 3 Bio (CONC3, 07-1114-4)

Use the recommended control material. Other suitable control material can also be used.
Control intervals and acceptance limits should be adapted to each lab\u00adoratory's individual requirements. If values do not fall within the defined limits, corrective measures and recalibration are required.
Conversion factors
Conversion factors for calcium concentration:
  • 1 mmol/L = 40.08 mg/L
  • 1 mg/L = 0.02495 mmol/L
Traceability
This method has been standardized against a primary standard material (SRM 956 c level 2).
Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ. Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 21 days).
 Level 1Level 2Level 3
Mean0.59 mmol/L (23.7 mg/L)2.53 mmol/L (101 mg/L)4.48 mmol/L (180 mg/L)
CV in-run [%]2.91.81.8
CV inter-run [%]3.51.92.1
", "Name": "Protocols" }, { "Language": "en", "Value": "Calcium is a mineral nutrient, which is reqired in cell cultures and microbial fermentation for optimal growth and product formation. For control of culture media and for monitoring in a culture process, the calcium concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Colorimetric detection with NM-BAPTA.
Recovery: ±10%", "Name": "Specification" } ] } } ] }

Calcium Bio HT

Test kit for the Cedex Bio HT Analyzer

cedex-calcium-bio-ht

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