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| Vial | Content | Composition |
|---|---|---|
| CA2B | Reagent cassette, 300 tests | Reagent 1 (R1): CAPSO, 557 mmol/L; pH 10.0; 5-nitro-5′-methyl-BAPTA (NM-BAPTA), 2 mmol/L; non-reactive surfactant; preservative. |
| Start Reagent (SR): EDTA, 7.5 mmol/L, pH 7.3; non-reactive surfactant; preservative. |
| Test definition | CA2B (745) | CA2D (790) |
|---|---|---|
| Measuring range mmol/L mg/L | Standard 0.2 – 5.0 8 – 200 | High 2 – 50(1) 80 – 2,000(1) |
| Control level | C 1, 2, 3 | C 3 |
| Sample predilution | No | 1:10 |
| Measuring mode | Absorbance | Absorbance |
| Abs. calc. mode | Endpoint | Endpoint |
| Reaction mode | R1-S-SR | R1-S-SR |
| Reaction direction | Decrease | Decrease |
| Wavelength A/B | 340/378 nm | 340/378 nm |
| Calc. first/last | 33/36 | 33/36 |
| Unit | mmol/L, mg/L | mmol/L, mg/L |
| Pipetting parameters | CA2B (745) | CA2D (790) |
|---|---|---|
| Water Diluent (Sample Predilution)(1) | No | 20 μL sample + 180 μL water |
| R1 | 20 μL + 130 μL water | 20 μL + 130 μL water |
| Sample (S) | 3 μL + 30 μL water | 3 μL + 30 μL water |
| SR | 20 μL | 20 μL |
| Total Volume | 203 μL | 203 μL |
| Calibration | |
|---|---|
| Calibrator | Calibrator C Bio (CAL C, 07-1110-1) |
| Calibration mode | Linear regression |
| Calibration interval | Each new lot, and if recalibration is required due to QC results. |
| Level 1 | Level 2 | Level 3 | |
|---|---|---|---|
| Mean | 0.59 mmol/L (23.7 mg/L) | 2.53 mmol/L (101 mg/L) | 4.48 mmol/L (180 mg/L) |
| CV in-run [%] | 2.9 | 1.8 | 1.8 |
| CV inter-run [%] | 3.5 | 1.9 | 2.1 |
Test kit for the Cedex Bio HT Analyzer
