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For further processing only. Others Biotin-16-UTP 10 mM solution Biotin-16-UTP 3.6.13.1.2.1 10 mM solution 11413201103 Biotin-16-UTP Biotin-16-UTP 08430215046692 Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as ''Biotin-16-UTP''. Unit of measure is ''nmol''. 86303-26-6 en Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA Polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling.Linearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and Biotin-16-UTP, respectively.The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Streptavidin-AP conjugate. en Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates. en Appearance: Clear, colorless solution Biotin-16-UTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 10.0-11.0 mmol/L Purity (HPLC): ≥85 area% Function test using DIG RNA Labeling Kit (SP6/T7) (Sensitivity Dot Blot ≤0.1 pg): Corresponds to reference Stability: At -15 to -25°C within specification range for 12 months. en