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Linearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and Biotin-16-UTP, respectively.
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by ELISA using a Streptavidin-AP conjugate.", "Name": "Applications" }, { "Language": "en", "Value": "Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates.", "Name": "Positioning" }, { "Language": "en", "Value": "Nomenclature: Biotin-16-uridine-5'-triphosphate
CAS Number: 86303-26-6
Formula: C32H48N7O19P3SLi4
Molecular weight: 987.5 D", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Biotin-16-UTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 10.0-11.0 mmol/L
Purity (HPLC): ≥85 area%
Function test using DIG RNA Labeling Kit (SP6/T7) (Sensitivity Dot Blot ≤0.1 pg): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" } ] } } ] }

Biotin-16-UTP

10 mM solution

Overview

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Technical documents

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