For further processing only. Others Biotin-16-ddUTP 1 mM solution Biotin-16-ddUTP 3.6.13.1.3.1 1 mM solution 11431692103 Biotin-16-ddUTP Biotin-16-ddUTP 07613336131008 Reagents, kits custom fill Not Available CustomBiotech product. Please contact your local representative. Will be supplied as ''Biotin-16-ddUTP''. Unit of measure is ''nmol''. 422268-45-9 en Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates. en Biotin-16-ddUTP is used as a substrate for:Terminal TransferaseDNA polymerase I (holoenzyme and Klenow fragment)T4 DNA polymeraseTaq DNA polymerase and reverse transcriptase (e.g.,Transcriptor)Biotin-16-ddUTP is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant.The biotin-labeled oligonucleotide can be used as a hybridization probe for:DNA and RNA transfersColony and plaque screeningIn situ hybridizationIn addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.Oligonucleotides are enzymatically labeled at their 3' end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine-triphosphate (biotin-ddUTP). en Appearance: Clear, colorless solution Biotin-16-ddUTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L Purity (HPLC): 85.0-100.0 area% Function test using DIG Oligonucleotide 3'-End-Labeling Kit, 2nd generation (DIG is replaced by Biotin-16-ddUTP): Corresponds to reference Stability: At -15 to -25°C within specification range for 24 months. en