{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Biotin-16-ddUTP 1 mM solution", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "Biotin-16-ddUTP", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.13.1.3.1", "ProductNameAddition": "1 mM solution", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates.", "Name": "Positioning" }, { "Language": "English", "Value": "Nomenclature: Biotin-16-2',3'-dideoxy-uridine-5'-triphosphate
Formula: C₃₂H₄₈N₇O₁₇P₃SLi₄
Molecular weight: 955.5 D", "Name": "CB - Properties" }, { "Language": "English", "Value": "Will be supplied as ''Biotin-16-ddUTP''. Unit of measure is ''nmol''.", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Biotin-16-ddUTP (1 μmol ≙ 10.7 A₂₄₀ units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test using DIG Oligonucleotide 3'-End-Labeling Kit, 2^nd generation (DIG is replaced by Biotin-16-ddUTP): Corresponds to reference
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Biotin-16-ddUTP is used as a substrate for:

• Terminal Transferase
• DNA polymerase I (holoenzyme and Klenow fragment)
• T4 DNA polymerase
• Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor)

Biotin-16-ddUTP is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant.
The biotin-labeled oligonucleotide can be used as a hybridization probe for:

• DNA and RNA transfers
• Colony and plaque screening
• In situ hybridization

In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors.
The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection.
Oligonucleotides are enzymatically labeled at their 3' end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine-triphosphate (biotin-ddUTP).", "Name": "Applications" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Biotin-16-ddUTP (1 μmol ≙ 10.7 A₂₄₀ units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test using DIG Oligonucleotide 3'-End-Labeling Kit, 2^nd generation (DIG is replaced by Biotin-16-ddUTP): Corresponds to reference
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" }, { "Language": "English", "Value": "Nomenclature: Biotin-16-2',3'-dideoxy-uridine-5'-triphosphate
Formula: C₃₂H₄₈N₇O₁₇P₃SLi₄
Molecular weight: 955.5 D", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Additional Applications", "StructureSystemID": null, "NodeID": "02-0073" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Labeled Nucleotides", "StructureSystemID": null, "NodeID": "70-030-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11431692103", "MaterialDescription": "Biotin-16-ddUTP", "RegisteredProductName": "", "GTIN": "07613336131008", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Biotin-16-ddUTP''. Unit of measure is ''nmol''." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }