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Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor)
Biotin-16-ddUTP is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant. The biotin-labeled oligonucleotide can be used as a hybridization probe for:
DNA and RNA transfers
Colony and plaque screening
In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors. The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection. Oligonucleotides are enzymatically labeled at their 3' end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine-triphosphate (biotin-ddUTP).",
"Country": "XG",
"Code": "Applications",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless solution Biotin-16-ddUTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L Purity (HPLC): 85.0-100.0 area% Function test using DIG Oligonucleotide 3'-End-Labeling Kit, 2nd generation (DIG is replaced by Biotin-16-ddUTP): Corresponds to reference Stability: At -15 to -25°C within specification range for 24 months.",
"Country": "XG",
"Code": "Specification",
"Name": "Specification"
},
{
"Language": "en",
"Value": "Nomenclature: Biotin-16-2',3'-dideoxy-uridine-5'-triphosphate CAS Number: 422268-45-9 Formula: C32H48N7O17P3SLi4 Molecular weight: 955.5 D",
"Country": "XG",
"Code": "Properties",
"Name": "Properties"
}
]
}
}
]
}
