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{
"ProductData": {
"RelatedLinks": "",
"RegulatoryDisclaimer1": "For further processing only.",
"RegulatoryDisclaimer2": "",
"StainLocalization": [
""
],
"ProductType": "Others",
"DisclaimerGroup2": null,
"ProductNameGlobal": "Biotin-16-ddUTP 1 mM solution",
"DisclaimerGroup1": "Non-IVD",
"ControlTissue": [
""
],
"BrandName": "Biotin-16-ddUTP",
"LicenseDisclaimers": [
],
"Isotypes": "",
"RegulatoryDisclaimer3": "",
"ID": "3.6.13.1.3.1",
"ProductNameAddition": "1 mM solution",
"Species": [
""
],
"Clone": ""
},
"ProductImageDetails": {
"ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png",
"ImageType": "Image main"
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"Product2Taxomony": {
"Product2TaxomonyReferences": [
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"StructureSystemIdentifier": "OWP_Product_Solutions",
"StructureSystemName": "OWP Product Solutions",
"NodeName": "CustomBiotech",
"NodeID": "520"
},
{
"StructureSystemIdentifier": "OWP_Product_Types",
"StructureSystemName": "OWP Product Types",
"NodeName": "Raw Materials->Labeled Nucleotides",
"NodeID": "70-030-00"
},
{
"StructureSystemIdentifier": "Product_Grouping",
"StructureSystemName": "Product Grouping",
"NodeName": "CustomBiotech->Molecular Raw Materials",
"NodeID": "02-0136"
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{
"StructureSystemIdentifier": "OWP_Organization",
"StructureSystemName": "OWP Organization",
"NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience",
"NodeID": "01-01-01-07-00"
},
{
"StructureSystemIdentifier": "OWP_Techniques",
"StructureSystemName": "OWP Techniques",
"NodeName": "RT-PCR/qRT-PCR",
"NodeID": "300-00"
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{
"StructureSystemIdentifier": "Product_Solutions",
"StructureSystemName": "Product Solutions",
"NodeName": "CustomBiotech",
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},
"Product2Materials": {
"P2MaterialReferences": [
{
"MaterialNum": "11431692103",
"MaterialDescription": "Biotin-16-ddUTP",
"RegisteredProductName": "Biotin-16-ddUTP",
"GTIN": "07613336131008",
"ProductCategoryText": "Reagents, kits",
"OldMaterialNumber": "",
"PackSizePIM360": "custom fill",
"PackSizeDescPIM360": "",
"MaterialAnnotation": "CustomBiotech product. Please contact your local representative.",
"ReadyForUse": "",
"OrderInformation": "Will be supplied as ''Biotin-16-ddUTP''. Unit of measure is ''nmol''."
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"Product2Products": {
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"ProductSpec": [
{
"ProductSpecVariant": {
"Chapters": [
{
"Language": "en",
"Value": "Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates.",
"Name": "Positioning"
},
{
"Language": "en",
"Value": "Will be supplied as ''Biotin-16-ddUTP''. Unit of measure is ''nmol''.",
"Name": "CB - Order Information"
},
{
"Language": "en",
"Value": "Biotin-16-ddUTP is used as a substrate for:
Terminal Transferase
DNA polymerase I (holoenzyme and Klenow fragment)
T4 DNA polymerase
Taq DNA polymerase and reverse transcriptase (e.g.,Transcriptor)
Biotin-16-ddUTP is preferentially used for 3'-end labeling of oligonucleotides with Terminal Transferase, recombinant. The biotin-labeled oligonucleotide can be used as a hybridization probe for:
DNA and RNA transfers
Colony and plaque screening
In situ hybridization
In addition to common hybridization techniques, biotin-labeled oligonucleotides are specifically useful for screening expression libraries for sequence-specific DNA-binding proteins, for example, transcription factors. The labeled oligomer can be subsequently detected by ELISA using the Streptavidin-AP conjugate for nucleic acid detection. Oligonucleotides are enzymatically labeled at their 3' end with Terminal Transferase by incorporation of a single biotin-labeled dideoxyuridine-triphosphate (biotin-ddUTP).",
"Name": "Applications"
},
{
"Language": "en",
"Value": "Appearance: Clear, colorless solution Biotin-16-ddUTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 1.0-1.1 mmol/L Purity (HPLC): 85.0-100.0 area% Function test using DIG Oligonucleotide 3'-End-Labeling Kit, 2nd generation (DIG is replaced by Biotin-16-ddUTP): Corresponds to reference Stability: At -15 to -25°C within specification range for 24 months.",
"Name": "Specification"
},
{
"Language": "en",
"Value": "Nomenclature: Biotin-16-2',3'-dideoxy-uridine-5'-triphosphate CAS Number: 422268-45-9 Formula: C32H48N7O17P3SLi4 Molecular weight: 955.5 D",
"Name": "Properties"
}
]
}
}
]
}