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CAS Number: 9029-44-1
Molecular weight: Approximately 140 kD
Isoelectric point: 5.0-6.0
Michaelis constant (Phosphate buffer, pH 5.6, +25°C):
L-ascorbate: 3 x 10-4 mol/L
Inhibitors: 4-chloromercuribenzoate, CN-, Na2S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
pH optimum: 5.6-7.0 (see figure)
Temperature dependence: See figure
pH stability: 6.5-9.0 (see figure)
Thermal stability: Up to +70°C (see figure)
Stability of the lyophilizate: Stable at +35°C for at least 3 weeks (see figure).
Stability in solution: See figure
Specificity: Several analogs of ascorbate react.
Remark: A decrease in activity of approximately 10% may occur.", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Turquoise lyophilizate
Solubility: Clear, slightly turquoise solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, L-ascorbate): ≥170 U/mg lyophilizate
Specific activity (+25°C): ≥1,700 U/mg protein
Protein (Biuret): 8-14 mg/100mg lyophilizate
Contaminants (+25°C, expressed as percentage of Ascorbate Oxidase activity):
Catalase: ≤0.2
Aspartate aminotransferase (AST/GOT): ≤0.0003
Alanine aminotransferase (ALT/GPT): ≤0.0005
Contaminating oxidases (FOX): ≤0.0002
Stability: At -15 to -25°C within specification range for 12 months. Keep tightly sealed.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }

Ascorbate Oxidase

from Cucurbita species, poor of AST/ALT, lyophilizate


Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
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