{ "ProductData": { "ID": "3.1.1.3.19.4", "ProductType": "Others", "BrandName": "Ascorbate Oxidase", "ProductNameAddition": "from Cucurbita species, poor of AST/ALT, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Ascorbate Oxidase poor of AST ALT" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Oxidases", "NodeID": "70-040-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials", "NodeID": "02-0011" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "valid", "NodeName": "Enzyme Testing", "NodeID": "06-00-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "valid", "NodeName": "Nitrogen Metabolism Testing", "NodeID": "16-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11136364103", "MaterialDescription": "Ascorbate Oxidase GOT-deficient", "RegisteredProductName": "Ascorbate Oxidase", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Ascorbate Oxidase GOT-deficient\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"Ascorbate Oxidase GOT-deficient\". Unit of measure is \"MU\".", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use Ascorbate Oxidase, poor of AST/ALT, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. It is useful in liquid as well as dry chemistry test, e.g., for the determination of uric acid, lactate, creatinine or transaminases.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Oxidoreductase that oxidizes ascorbic acid to dehydroascorbate.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Nomenclature: L-ascorbate:oxygen oxidoreductase
CAS Number: 9029-44-1
Molecular weight: Approximately 140 kD
Isoelectric point: 5.0-6.0
Michaelis constant (Phosphate buffer, pH 5.6, +25°C):
L-ascorbate: 3 x 10^-4 mol/L
Inhibitors: 4-chloromercuribenzoate, CN^-, Na₂S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
pH optimum: 5.6-7.0 (see figure)
Temperature dependence: See figure
pH stability: 6.5-9.0 (see figure)
Thermal stability: Up to +70°C (see figure)
Stability of the lyophilizate: Stable at +35°C for at least 3 weeks (see figure).
Stability in solution: See figure
Specificity: Several analogs of ascorbate react.
Remark: A decrease in activity of approximately 10% may occur.", "Country": "XG", "Code": "Properties", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Turquoise lyophilizate
Solubility: Clear, slightly turquoise solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, L-ascorbate): ≥170 U/mg lyophilizate
Specific activity (+25°C): ≥1,700 U/mg protein
Protein (Biuret): 8-14 mg/100mg lyophilizate
Contaminants (+25°C, expressed as percentage of Ascorbate Oxidase activity):
Catalase: ≤0.2
Aspartate aminotransferase (AST/GOT): ≤0.0003
Alanine aminotransferase (ALT/GPT): ≤0.0005
Contaminating oxidases (FOX): ≤0.0002
Stability: At -15 to -25°C within specification range for 12 months. Keep tightly sealed.", "Country": "XG", "Code": "Specification", "Name": "Specification" } ] } } ] }