{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Ascorbate Oxidase", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Ascorbate Oxidase", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.1.3.19.1", "ProductNameAddition": "from Cucurbita species, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Oxidases", "NodeID": "70-040-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Clinical Chemistry Raw Materials", "NodeID": "02-0011" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Carbohydrate Testing", "NodeID": "01-00-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "Lipid Testing", "NodeID": "11-00-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10199605103", "MaterialDescription": "Ascorbate Oxidase from Cucurbita species", "RegisteredProductName": "Ascorbate Oxidase", "GTIN": "14038377155316", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Ascorbate Oxidase from Cucurbita species''. Unit of measure is ''kU''." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Oxidoreductase that oxidizes ascorbic acid to dehydroascorbate.", "Name": "Positioning" }, { "Language": "en", "Value": "Will be supplied as ''Ascorbate Oxidase from Cucurbita species''. Unit of measure is ''kU''.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use Ascorbate Oxidase in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. It is useful in liquid as well as dry chemistry test, e.g., for the determination of uric acid, lactate or creatinine.", "Name": "Applications" }, { "Language": "en", "Value": "Appearance: Turquoise lyophilizate
Solubility: Clear, slightly turquoise solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, L-ascorbate): ≥170 U/mg lyophilizate
Specific activity (+25°C): ≥1,700 U/mg protein
Protein (Biuret): 0.07-0.14 mg/mg lyophilizate
Contaminants (+25°C; expressed as percentage of Ascorbate Oxidase activity):
Catalase: ≤0.2
Aspartate aminotransferase (AST/GOT): No limit
Alanine aminotransferase (ALT/GPT): No limit
Peroxidase: ≤0.005
Stability: At -15 to -25°C within specification range for 12 months. Keep tightly sealed.", "Name": "Specification" }, { "Language": "en", "Value": "Nomenclature: L-ascorbate:oxygen oxidoreductase
CAS Number: 9029-44-1
Molecular weight: Approximately 140 kD
Isoelectric point: 5.0-6.0
Michaelis constant (Phosphate buffer, pH 5.6, +25°C):
L-ascorbate: 3 x 10-4 mol/L
Inhibitors: 4-chloromercuribenzoate, CN-, Na2S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
pH optimum: 5.6-7.0 (see figure)
Temperature dependence: See figure
pH stability: 6.5-9.0 (see figure)
Thermal stability: Up to +70°C (see figure)
Stability of the lyophilizate: Stable at +35°C for at least 3 weeks (see figure).
Stability in solution: See figure
Specificity: Several analogs of ascorbate react.
Remark: A decrease in activity of approximately 10% may occur.", "Name": "Properties" } ] } } ] }

Ascorbate Oxidase

from Cucurbita species, lyophilizate

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