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05890152103: Will be supplied as ''AptaTaq Genotyping Master''. Unit of measure is ''mL''.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Product Description", "Value": "AptaTaq DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for the amplification of difficult sample types, reaction buffer, and a dNTP mix using dUTP instead of dTTP (for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase).", "Language": "en", "Country": "XG", "Code": "Product Description" }, { "Name": "Benefits", "Value": "
  • Reduce time to result.
    Save up to 15 minutes per run by omitting the initial activation step required by chemically modified hot start polymerases, and reduce cycling time with fast protocols.
  • Ready for robotics.
    Rely on the stability of the AptaTaq Genotyping Master mix for PCR automation. The viscosity of the master mix is optimized for accurate pipetting. The mix is stable during setup and on the stacker for more than 24 hours.
  • Gain flexibility.
    The 5x concentrated master mix enables you to vary reaction volume and sample input for outstanding results. Use AptaTaq Genotyping Master mix for all real-time PCR instruments not requiring Rox normalization. For instruments requiring Rox normalization, use AptaTaq Genotyping Master (Rox).
  • Benefit from high stability.
    Keep the master mix in the refrigerator for up to 4 weeks and profit from a quick setup without thawing first.
", "Language": "en", "Country": "XG", "Code": "Benefits" }, { "Name": "Background Information", "Value": "The aptamer/polymerase mixture is a hot start system with reversible inhibition of the polymerase activity at lower temperatures. Polymerase inactivation is achieved by a tight bond of the folded aptamer-oligonucleotide to the active site of the polymerase at lower temperatures. Upon heating above +60°C, the aptamer acts like a molecular switch, changing its temperature-dependent tertiary structure and releasing the active polymerase. Dropping the temperature below +55°C shuts off the polymerase activity again. Similar to antibody-based methods, the enzyme is much more quickly activated by heating, than chemically modified polymerases. In contrast to antibodies, the aptamer-oligonucleotide is much more stable, allowing longer storage at room temperature.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Positioning", "Value": "Reversible hot start DNA master mix without initial activation step for maximum stability combined with sensitivity and specificity; lyo ready formulation for preparation of dried amplification mixes.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Performance test in qPCR using ABI 7500
(human genomic DNA, CycA fragment): Corresponds to specification
(human genomic DNA, β-globin fragment): Corresponds to specification
(human genomic DNA, ApoE fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "The master mix is very stable and can be stored in the refrigerator (+2 to +8°C) for at least 4 weeks without loss of activity and performance. It is stable at room temperature for at least 2 days.", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }

AptaTaq Genotyping Master

5x concentrated