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"Name": "Applications",
"Value": "Use AptaTaq exo DNA Polymerase for:
- SNP analysis and genotyping
- Allele-specific PCR
- Multiplexing
- Arbitrarily primed PCR
- Formulation of dried-down amplification reagents
When time to result matters, this novel hot start technology is ideal as it does not require any activation time.",
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"Name": "Product Description",
"Value": "AptaTaq DNA exo Polymerase is a blend of N-terminal truncated Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for excellent discrimination against misextension. This concentrated formulation contains no glycerol and is suitable for the preparation of dried-down amplification mix preparations.",
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"Name": "Benefits",
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- Optimize your SNP analysis.
Discriminate between paired and unpaired primer ends using an enzyme optimized for allele-specific PCR. - Obtain reliable results fast.
Benefit from the general features of the AptaTaq DNA Polymerase System with the discriminating capabilities of a 5'-3' exonuclease activity-lacking Taq DNA Polymerase. - Prepare stable amplification mixes in dry format.
Use this formulation for producing dried-down amplification mixes stable at room temperature.
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{
"Name": "Background Information",
"Value": "The aptamer/polymerase mixture is a hot start system with reversible inhibition of the polymerase activity at lower temperatures. Polymerase inactivation is achieved by a tight bond of the folded aptamer-oligonucleotide to the active site of the polymerase at lower temperatures. Upon heating above +60°C, the aptamer acts like a molecular switch, changing its temperature-dependent tertiary structure and releasing the active polymerase. Dropping the temperature below +55°C shuts off the polymerase activity again. Similar to antibody-based methods, the enzyme is much more quickly activated by heating, than chemically modified polymerases. In contrast to antibodies, the aptamer-oligonucleotide is much more stable, allowing longer storage at room temperature.",
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"Value": "N-terminal truncated Taq DNA Polymerase with reversible hot start system and no 5'-3' exonuclease activity for optimal detection of mismatches;
lyo ready formulation for preparation of dried amplification mixes.",
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"Name": "Properties",
"Value": "AptaTaq exoDNA Polymerase is active at temperatures above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase itself is a highly processive 5'-3' DNA Polymerase lacking 5'-3' and 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 8.3 (+20°C)
Temperature optimum for elongation: Approximately +72°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg
2+ (standard concentration, 2 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP",
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Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L;EDTA, 0.1 mmol/L; DTT, 1 mmol/L; casein 1 g/L; glycerol-free; pH approximately 8.0 at +4°C
Volume activity: 55±5 U/μL
Aptamer concentration (HPLC): 240 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Function test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.",
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