{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "AptaTaq exo DNA Polymerase 5 U uL", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "AptaTaq exo DNA Polymerase, 5 U/μl", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.6.17.2.1.13", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Solutions", "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Family", "NodeName": "AptaTaq", "NodeID": "480" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials->DNA Polymerases", "NodeID": "70-042-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "OWP Techniques", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "05458030103", "MaterialDescription": "AptaTaq delta exo DNA Polymerase, 5 U/ul", "RegisteredProductName": "AptaTaq exo DNA Polymerase, 5 U/?L", "GTIN": "07613336130827", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "5 U/μL", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"AptaTaq exo DNA Polymerase, 5 U/μL\". Unit of measure is \"kU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "This novel optimized mixture of high-quality N-terminal-deleted Taq DNA Polymerase and a specific oligonucleotide (aptamer) provides improved discrimination against misextension. As with the AptaTaq DNA Polymerase System, the AptaTaq exo DNA Polymerase-based assay shows high specificity and a broad dynamic range of products.", "Name": "Product Description" }, { "Language": "en", "Value": "Use AptaTaq exo DNA Polymerase for:

• SNP analysis and genotyping
• Allele-specific PCR
• Multiplexing
• Arbitrarily primed PCR
• Automated PCR requiring prolonged handling at room temperature

When time to result matters, this novel hot start technology is ideal as it does not require any activation time.", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"AptaTaq exo DNA Polymerase, 5 U/μL\". Unit of measure is \"kU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "N-terminal truncated Taq DNA Polymerase with reversible hot start system and no 5'-3' exonuclease activity for optimal detection of mismatches.", "Name": "Positioning" }, { "Language": "en", "Value": "

• Optimize your SNP analysis.
  Discriminate between paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
• Obtain reliable results fast.
  Benefit from the general features of the AptaTaq DNA Polymerase System with the differentiating capabilities of a 5'-3' exonuclease activity-lacking Taq DNA Polymerase.

", "Name": "Benefits" }, { "Language": "en", "Value": "The aptamer/polymerase mixture is a hot start system with reversible inhibition of the polymerase activity at lower temperatures. Polymerase inactivation is achieved by a tight bond of the folded aptamer-oligonucleotide to the active site of the polymerase at lower temperatures. Upon heating above +60°C, the aptamer acts like a molecular switch, changing its temperature-dependent tertiary structure and releasing the active polymerase. Dropping the temperature below +55°C shuts off the polymerase activity again. Similar to antibody-based methods, the enzyme is much more quickly activated by heating, than chemically modified polymerases. In contrast to antibodies, the aptamer-oligonucleotide is much more stable, allowing longer storage at room temperature.", "Name": "Background Information" }, { "Language": "en", "Value": "AptaTaq exo DNA Polymerase is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase that lacks 5'-3' and 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 8.3 (+20°C)
Temperature optimum for elongation: Approximately +72°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg²⁺ (standard concentration, 2 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); casein, 0.1 g/L; pH approximately 8.0 at +4°C
Volume activity: 5.5±0.5 U/μL
Aptamer concentration (HPLC): 24.0 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (³H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Performance test in qPCR using LightCycler^® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" } ] } } ] }