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"RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.",
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"ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, solution",
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"MaterialDescription": "AptaTaq delta exo DNA Polymerase, 5 U/ul",
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"Chapters": [
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"Language": "en",
"Value": "This novel optimized mixture of high-quality N-terminal-deleted Taq DNA Polymerase and a specific oligonucleotide (aptamer) provides improved discrimination against misextension. As with the AptaTaq DNA Polymerase System, the AptaTaq exo DNA Polymerase-based assay shows high specificity and a broad dynamic range of products.",
"Name": "Product Description"
},
{
"Language": "en",
"Value": "Use AptaTaq exo DNA Polymerase for:
SNP analysis and genotyping
Allele-specific PCR
Multiplexing
Arbitrarily primed PCR
Automated PCR requiring prolonged handling at room temperature
When time to result matters, this novel hot start technology is ideal as it does not require any activation time.",
"Name": "Applications"
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{
"Language": "en",
"Value": "Will be supplied as \"AptaTaq exo DNA Polymerase, 5 U/μL\". Unit of measure is \"kU\".",
"Name": "CB - Order Information"
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{
"Language": "en",
"Value": "N-terminal truncated Taq DNA Polymerase with reversible hot start system and no 5'-3' exonuclease activity for optimal detection of mismatches.",
"Name": "Positioning"
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"Value": "
Optimize your SNP analysis. Discriminate between paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
Obtain reliable results fast. Benefit from the general features of the AptaTaq DNA Polymerase System with the differentiating capabilities of a 5'-3' exonuclease activity-lacking Taq DNA Polymerase.
",
"Name": "Benefits"
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"Language": "en",
"Value": "The aptamer/polymerase mixture is a hot start system with reversible inhibition of the polymerase activity at lower temperatures. Polymerase inactivation is achieved by a tight bond of the folded aptamer-oligonucleotide to the active site of the polymerase at lower temperatures. Upon heating above +60°C, the aptamer acts like a molecular switch, changing its temperature-dependent tertiary structure and releasing the active polymerase. Dropping the temperature below +55°C shuts off the polymerase activity again. Similar to antibody-based methods, the enzyme is much more quickly activated by heating, than chemically modified polymerases. In contrast to antibodies, the aptamer-oligonucleotide is much more stable, allowing longer storage at room temperature.",
"Name": "Background Information"
},
{
"Language": "en",
"Value": "AptaTaq exo DNA Polymerase is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase that lacks 5'-3' and 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). The enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates. pH optimum: Approximately 8.3 (+20°C) Temperature optimum for elongation: Approximately +72°C Half life at +95°C: Approximately 40 minutes Divalent ion requirement: Mg2+ (standard concentration, 2 mmol/L) dNTP requirement: Approximately 200 μmol/L for each dNTP",
"Name": "Properties"
},
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"Language": "en",
"Value": "Appearance: Clear, colorless solution Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); casein, 0.1 g/L; pH approximately 8.0 at +4°C Volume activity: 5.5±0.5 U/μL Aptamer concentration (HPLC): 24.0 μmol/L ±10% Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C. Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C. Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference Stability: At -15 to -25°C within specification range for 12 months.",
"Name": "Specification"
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}
AptaTaq exo DNA Polymerase, 5 U/μl
from Thermus aquaticus BM, expressed in E. coli, solution