{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "AptaTaq DNA Polymerase LDx 50 U uL", "DisclaimerGroup1": null, "ControlTissue": [ "" ], "BrandName": "AptaTaq DNA Polymerase LDx, 50 U/μl", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.", "ID": "3.6.17.2.1.11", "ProductNameAddition": "from Thermus aquaticus BM, expressed in E. coli, glycerol-free solution", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Will be supplied as \"AptaTaq DNA Polymerase. LDx, Glyc.-free, 50 U/μL\". Unit of measure is \"kU\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Select AptaTaq DNA Polymerase LDx to perform microbial testing and other assays where the absence of contaminating bacterial, fungal, and/or human DNA is crucial. AptaTaq DNA LDx Polymerase is ideal for:

• Fast PCR assays with no extra enzyme activation time and fast cycling protocols
• Single- and multiplex PCR and qPCR applications that require high specificity, sensitivity, and yield
• RT-PCR
• Difficult templates with complex secondary structures or GC-rich sequences
• Formulation of dried-down amplification reagents

", "Name": "Applications" }, { "Language": "English", "Value": "AptaTaq DNA Polymerase LDx is a blend of Taq DNA Polymerase and a specific oligonucleotide (aptamer) with hot start features, optimized for applications detecting the lowest levels of DNA.", "Name": "Product Description" }, { "Language": "English", "Value": "AptaTaq DNA Polymerase LDx is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase lacking 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). This enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum for elongation: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg²⁺ (standard concentration, 1.5 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP", "Name": "CB - Properties" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Volume activity: 55±5 U/μL
Glycerol content: ≤0.1% (v/v)
Aptamer concentration (HPLC): 35.75 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (³H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Tests for the presence of contaminating nucleic acids
(human genomic DNA, β-Globin fragment): Corresponds to specification
(LC UniTool Resolight assay, specific for grampositive and gramnegative bacterial DNA and fungi DNA, <1.0 copy genomic DNA/20 U enzyme): Corresponds to specification
Performance test in qPCR using LightCycler^® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "

• Minimize risk of contaminating nucleic acids.
  AptaTaq DNA Polymerase LDx is extensively evaluated using ultra sensitive tests for detecting contaminating nucleic acids from bacteria and fungi. Roche has developed a nucleic acid-free workflow with clearly defined, highly consistent manufacturing processes resulting in a product with very low nucleic acid background.

• Prepare stable amplification mixes in dry format.Use this formulation for producing dried-down amplification mixes stable at room temperature.

• Enjoy the benefits of the advanced AptaTaq hot start system.
  Refer to AptaTaq DNA Polymerase for additional benefits like speed, easy handling and consistent results.

", "Name": "Benefits" }, { "Language": "English", "Value": "Reversible hot start Taq DNA Polymerase without initial activation step for maximum stability combined with sensitivity and specificity in microbial testing; lyo ready formulation for preparation of dried amplification mixes.", "Name": "Positioning" }, { "Language": "English", "Value": "LDx quality contains a very low DNA background, as verified using an ultra-sensitive LightCycler^® assay for the absence of gram(+), gram(-) bacteria, and fungal DNA. To pass this Quality Control test, the level of contaminating nucleic acid must be <1 genome equivalent per 20 units of DNA polymerase. Furthermore, it is analyzed for the absence of contaminating human DNA with a LightCycler^® test, specific for β-globin.", "Name": "Quality Control" }, { "Language": "English", "Value": "For information on LDx refer to AptaTaq DNA Polymerase LDx, 5 U/μl
For additional information on the AptaTaq hot start system, see AptaTaq DNA Polymerase, 5 U/μl", "Name": "Background Information" }, { "Language": "English", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); pH approximately 8.0 at +4°C
Volume activity: 55±5 U/μL
Glycerol content: ≤0.1% (v/v)
Aptamer concentration (HPLC): 35.75 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (³H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Tests for the presence of contaminating nucleic acids
(human genomic DNA, β-Globin fragment): Corresponds to specification
(LC UniTool Resolight assay, specific for grampositive and gramnegative bacterial DNA and fungi DNA, <1.0 copy genomic DNA/20 U enzyme): Corresponds to specification
Performance test in qPCR using LightCycler^® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" }, { "Language": "English", "Value": "AptaTaq DNA Polymerase LDx is active at temperature above +60 to +65°C and inactive below +55°C. This hot start feature eliminates the risk of nonspecific primer extension. Taq DNA Polymerase is a highly processive 5'-3' DNA Polymerase lacking 3'-5' exonuclease activity. Taq DNA Polymerase is stable during prolonged incubations at elevated temperatures (+95°C). This enzyme exhibits highest activity at a pH of approximately 9 (adjusted at +20°C) and temperatures approximately +75°C. Taq DNA Polymerase accepts dNTP analogs as substrates.
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum for elongation: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Divalent ion requirement: Mg²⁺ (standard concentration, 1.5 mmol/L)
dNTP requirement: Approximately 200 μmol/L for each dNTP", "Name": "Properties" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Family", "NodeName": "AptaTaq", "StructureSystemID": null, "NodeID": "480" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->DNA Polymerases", "StructureSystemID": null, "NodeID": "70-042-00" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "05447895103", "MaterialDescription": "AptaTaq DNA Polymerase LDx, 50U/ul", "RegisteredProductName": "", "GTIN": "07613336130889", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"AptaTaq DNA Polymerase. LDx, Glyc.-free, 50 U/μL\". Unit of measure is \"kU\"." } ] }, "Product2Products": { "Product2ProductReference": [ { "ProductID": "3.6.17.2.1.20", "BrandName": "AptaTaq Fast PCR Buffer", "ProductNameAddition": "5x concentrated", "ReferenceType": "Reagent" } ] } }