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Unit of measure is \"kU\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Apply AptaTaq DNA Polymerase for:
  • Fast PCR assays with no extra enzyme activation time and fast cycling protocols
  • Single- and multiplex PCR and qPCR applications that require high specificity, sensitivity, and yield
  • RT-PCR
  • Difficult templates, such as complex secondary structures or GC-rich sequences
  • Automated PCR workflows requiring high stability of the reaction mixtures during automated pipetting and prolonged handling at room temperature
", "Name": "Applications" }, { "Language": "en", "Value": "Reversible hot start Taq DNA Polymerase without initial activation step for maximum stability combined with sensitivity and specificity.", "Name": "Positioning" }, { "Language": "en", "Value": "
  • Reduce time to result.
    Save up to 15 minutes per run by omitting the initial activation step required by chemically modified hot start polymerases, and reduce cycling time with fast protocols.
  • Maximize specificity, sensitivity, and yield.
    Achieve reliable amplification of your target DNA from various sources (e.g., genomic DNA, cDNA, plasmids).
  • Simplify PCR setup.
    Store these highly stable polymerase for up to 1 month at +2° to +8°C and set up your hot start PCR reaction at room temperature.
", "Name": "Benefits" }, { "Language": "en", "Value": "The aptamer/polymerase mixture is a hot start system with reversible inhibition of the polymerase activity at lower temperatures. Polymerase inactivation is achieved by a tight bond of the folded aptamer-oligonucleotide to the active site of the polymerase at lower temperatures. Upon heating above +60°C, the aptamer acts like a molecular switch, changing its temperature-dependent tertiary structure and releasing the active polymerase. Dropping the temperature below +55°C shuts off the polymerase activity again. Similar to antibody-based methods, the enzyme is much more quickly activated by heating, than chemically modified polymerases. In contrast to antibodies, the aptamer-oligonucleotide is much more stable, allowing longer storage at room temperature.", "Name": "Background Information" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; EDTA, 0.1 mmol/L; DTT, 1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50.0% (v/v); pH approximately 8.0 at +4°C
Volume activity: 5.5±0.5 U/μL
Aptamer concentration (HPLC): 3.58 μmol/L ±10%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 30 U after 4 hours incubation at +65°C.
Performance test in qPCR using LightCycler® 480 (≥0.03 ng human genomic DNA, 339 bp tPA fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.", "Name": "Specification" }, { "Language": "en", "Value": "AptaTaq DNA Polymerase is reversibly inhibited below +55°C and becomes active at temperatures over +60°C. This hot start feature eliminates the risk of nonspecific primer extension during PCR setup.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand-specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Activation temperature: Active at ≥+60°C
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP and various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants).
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)", "Name": "Properties" } ] } } ] }

AptaTaq DNA Polymerase, 5 U/μl

from Thermus aquaticus BM, expressed in E. coli, solution

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