For further processing only. Others alpha-2 6-Sialyltransferase rec. EQ α-2,6-Sialyltransferase, rec. EQ 78 1403506419168 The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product. 220.127.116.11.13.3 human, expressed in CHO-K1 cells alpha-2,6-Sialyltransferase, rec. EQ custom fill CustomBiotech product. Please contact your local representative. Unit of measure is "gram". alpha-2,6-Sialyltransferase, rec. EQ 08098174103 Not Available Reagents, kits en α-2,6-Sialyltansferase, rec. EQ is a highly active recombinant human glycosyltransferase, expressed in CHO cells.To use in vitro glycoengineering in drug development and manufacturing processes, enzymes should be of enhanced quality (EQ), compared to enzymes used in early R&D or analytical applications: Enzymes should be tested for host-cell impurities and relevant metal impurities and be available at least in gram amounts .α-2,6-Sialyltansferase, rec. EQ fulfills those criteria and can also be provided in GMP Grade upon request. en Incubation of α-2,6-Sialyltansferase and CMP-NANA with a glycoprotein leads to increased sialylation of the glycoprotein.As α-2,6-Sialyltansferase rec. EQ adds sialic acid only to galactosylated glycostructures, a galactosylation of the glycoprotein in a previous step helps to achieve higher sialylation levels. Galactosylation can be achieved by incubation of the target protein with ß-1,4-Galactosyltransferase and UDP-Galactose. en α-2,6-Sialyltransferase, rec. EQ can be used for in vitro glycoengineering of glycoproteins, e.g. therapeutic proteins, in drug development and manufacturing. en Benefits of using in vitro glycoengineering to modify glycosylation of therapeutic proteins: Time- and cost-saving generation of glycan variants to facilitate drug development. Optimized glycosylation without compromising other CQAs or product yield. Improved lot-to-lot consistency to reduce risk of product quality variation and resulting delays. Generation of glycoprofiles which may not otherwise be generated. Streamlined analytics in comparability studies. en Appearance: Clear and colorless to slightly yellowish solutionSpecific activity: ≥400 U/μgProtein (OD 280 nm) A280 = 1.931: 15 ± 1.5 mg/mLPurity (HPLC): >90%Bioburden: ≤100 CFU/mLEndotoxin: ≤10 EU/mgHost cell protein: ≤ 4000 ppmHost cell DNA: ≤ 100 pg/mgHeavy metal (Cd, Co, V, Ni, Ag, Se): ≤ 10 ppmStability: At –15 to –25°C within specification range for 18 months (08 098 174 103) and 12 months (08 098 174 101).