{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Alkaline Phosphatase recombinant highly active solution", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Alkaline Phosphatase, recombinant, highly active", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.7.3.1.1.13", "ProductNameAddition": "from calf intestine, expressed in Pichia pastoris, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Immunology Raw Materials", "NodeID": "02-0035" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "In vitro Glycoengineering", "NodeID": "02-0052" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Phosphatases", "NodeID": "70-041-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03137031103", "MaterialDescription": "AP,Yeast,high act.,rec.,EIA,NaCl", "RegisteredProductName": "Alkaline Phosphatase, recombinant, highly active", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"AP, Yeast, high act., rec., EIA, NaCl\". Unit of measure is \"g\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Marker enzyme for preparation of antibody- / antigen-enzyme conjugates incorporated in highly sensitive immunoassay reagents for colorimetric, fluorimetric and luminometric detection. Alkaline Phosphatase is recommended for conjugation via carbohydrate groups (content approximately 30%).", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"AP, Yeast, high act., rec., EIA, NaCl\". Unit of measure is \"g\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Alkaline Phosphatase recombinant, highly active catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, sugar alcohols, cyclic alcohols, phenols and amines. Phosphodiesters do not react with Alkaline Phosphatase, recombinant. The enzyme hydrolyzes PPi. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.", "Name": "Background Information" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution in NaCl, 3 mol/L; MgCl2, 5 mmol/L; ZnCl2, 0.1 mmol/L; Tea, 30 mmol/L, pH approximately 7.6
pH value: 7.0-8.0
Protein (A280, 1 mg/mL=1, against water): 20±1 mg/mL
Specific activity (+37°C, 4-NPP): ≥7,000 U/mg
Alkaline Phosphatase (HPLC): ≥95 area% (HPLC profile added to certificate)
Amino groups: 5-13 mol/mol
Carbohydrates, n=2: No limit
Stability: At +2 to +8°C within specification range for 12 months.", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number: 9001-78-9
IEP (IEF, CE): 3.6-4.7
MALDI-TOF MS:
Total molecular weight: 124±10 kD
Molecular weight protein: 104 kD (=84%)
Molecular weight carbohydrate: 20±10 kD (16±6%)
Accessible N-glycosylation sites: 2/subunit
Branching type: Higher branched (hybrid) type GlcNAc, Mannose, no NeuAc detected
O-glycosylation sites: Not detected
Number of isoenzymes:
Present: 3 (MS)
Based on protein: 1 (MS)", "Name": "Properties" } ] } } ] }

Alkaline Phosphatase, recombinant, highly active

from calf intestine, expressed in Pichia pastoris, solution

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