{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Alkaline Phosphatase recombinant highly active carbohydrate reduced", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Alkaline Phosphatase, recombinant, highly active, carbohydrate reduced", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.7.3.1.1.16", "ProductNameAddition": "from calf intestine, expressed in Pichia pastoris, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Immunology Raw Materials", "NodeID": "02-0035" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Phosphatases", "NodeID": "70-041-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "03535452103", "MaterialDescription": "AP,highly active, recombinant, CR", "RegisteredProductName": "Alkaline Phosphatase, recombinant, highly active, carbohydrate reduced", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"AP, highly active, recombinant, CR\". Unit of measure is \"g\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Marker enzyme for preparation of antibody- / antigen- enzyme conjugates incorporated in highly sensitive immunoassay reagents for colorimetric, fluorimetric and luminometric detection. Recommended for conjugation via amino groups.", "Name": "Applications" }, { "Language": "en", "Value": "Will be supplied as \"AP, highly active, recombinant, CR\". Unit of measure is \"g\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "AP recombinant, highly active CR catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, sugar alcohols, cyclic alcohols, phenols and amines. Phosphodiesters do not react with Alkaline Phosphatase, recombinant. The enzyme hydrolyzes PPi. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH and others. The product contains a significantly reduced carbohydrate moiety.", "Name": "Background Information" }, { "Language": "en", "Value": "Appearance: Clear, colourless solution in NaCl, 3 mol/L; MgCl2, 5 mmol/L; ZnCl2, 0.1 mmol/L; Tea, 30 mmol/L, pH approximately 7.6
pH value: 7.0-8.0
Protein (A280; 1 mg/mL=1; against water): 20±1 mg/mL
Specific activity (+37°C, 4-NPP): ≥ 7000 U/mg
Alkaline Phosphatase (HPLC): ≥95 area% (HPLC profile added to certificate)
Amino groups: 5-13 mol/mol
Carbohydrates, n=2: ≤7%
Stability: At +2 to +8°C within specification range for 12 months.", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number: 9001-78-9
IEP (IEF, CE): 5.2-6.0
MALDI-TOF MS:
Total molecular weight: 111 kD
Molecular weight protein: 104 kD (= 94%)
Molecular weight carbohydrates: 6.5 kD (= 6%)
Accessible N-glycosylation sites: 2/subunit
Branching type: Reduced branched type GlcNAc, Mannose no NeuAc detected
O-glycosylation sites: Not detected
Number of isoenzymes:
Present: 1 (MS)
Based on protein: 1 (MS)", "Name": "Properties" } ] } } ] }

Alkaline Phosphatase, recombinant, highly active, carbohydrate reduced

from calf intestine, expressed in Pichia pastoris, solution

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