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"ProductType": "Others",
"BrandName": "Alkaline Phosphatase, recombinant, 20 U/μL",
"ProductNameAddition": "from bovine intestine, expressed in
Pichia pastoris, solution",
"RegulatoryDisclaimer1": "For further processing only.",
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"RegulatoryDisclaimer3": null,
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"Clone": "",
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""
],
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""
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"ProductNameGlobal": "Alkaline Phosphatase recombinant 20 U uL"
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"StructureNodeStatus": "valid",
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"MaterialNum": "04571363103",
"MaterialDescription": "Alkaline Phosphatase rec.,MB Grade, 20 U",
"RegisteredProductName": "Alkaline Phosphatase, recombinant, 20 U/uL",
"GTIN": "07613336130711",
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"PackSizeDescPIM360": "20 U/μL",
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"OrderInformation": "Will be supplied as \"Alkaline Phosphatase rec., MB Grade, 20 U\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer."
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"ProductSpecVariant": {
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"Name": "CB - Order Information",
"Value": "Will be supplied as \"Alkaline Phosphatase rec., MB Grade, 20 U\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.",
"Language": "en",
"Country": "XG",
"Code": "CB - Order Information"
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{
"Name": "Positioning",
"Value": "Alkaline Phosphatase for 5' dephosphorylation of DNA and RNA.",
"Language": "en",
"Country": "XG",
"Code": "Positioning"
},
{
"Name": "Applications",
"Value": "Use Alkalkine Phosphatase, recombinant for:
- Dephosphorylation of 5' phosphate from DNA and RNA
- Coupling to other proteins via its amino or carbohydrate groups, for detection in immunoassays and western blot analysis
",
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"Country": "XG",
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"Value": "
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; MgCl
2, 1 mmol/L; ZnCl
2, 0.1 mmol/L; glycerol, 50% (v/v); pH approximately 7.6 at +4°C
Volume activity: ≥20 U/μL
Specific activity: ≥5 kU/mg protein
Unspecific endonucleases (λDNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 100 U after 1 hour incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.",
"Language": "en",
"Country": "XG",
"Code": "Specification"
},
{
"Name": "Properties",
"Value": "
CAS Number: 9001-78-9
Enzyme activity: Alkaline Phosphatase, recombinant, 1 U/μL catalyzes the dephosphorylation of 5' phosphate from DNA and RNA.
pH activity optimum: 9.8
pH stability optimum: 8.0
Cofactor: Zn
2+Aktivators: Mg
2+, Mn
2+, Co
2+Inactivation: Complete inactivation after 5 minutes at +75°C.",
"Language": "en",
"Country": "XG",
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