{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Alkaline Phosphatase recombinant 20 U uL", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Alkaline Phosphatase, recombinant, 20 U/μL", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.14.1.3.3", "ProductNameAddition": "from bovine intestine, expressed in Pichia pastoris, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Phosphatases", "NodeID": "70-041-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "NOT APPLICABLE", "NodeID": "999-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "04571363103", "MaterialDescription": "Alkaline Phosphatase rec.,MB Grade, 20 U", "RegisteredProductName": "Alkaline Phosphatase, recombinant, 20 U/uL", "GTIN": "07613336130711", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "20 U/μL", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Alkaline Phosphatase rec., MB Grade, 20 U\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as \"Alkaline Phosphatase rec., MB Grade, 20 U\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Alkaline Phosphatase for 5' dephosphorylation of DNA and RNA.", "Name": "Positioning" }, { "Language": "en", "Value": "Use Alkalkine Phosphatase, recombinant for:
  • Dephosphorylation of 5' phosphate from DNA and RNA
  • Coupling to other proteins via its amino or carbohydrate groups, for detection in immunoassays and western blot analysis
", "Name": "Applications" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; MgCl2, 1 mmol/L; ZnCl2, 0.1 mmol/L; glycerol, 50% (v/v); pH approximately 7.6 at +4°C
Volume activity: ≥20 U/μL
Specific activity: ≥5 kU/mg protein
Unspecific endonucleases (λDNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 100 U after 1 hour incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" }, { "Language": "en", "Value": "CAS Number: 9001-78-9
Enzyme activity: Alkaline Phosphatase, recombinant, 1 U/μL catalyzes the dephosphorylation of 5' phosphate from DNA and RNA.
pH activity optimum: 9.8
pH stability optimum: 8.0
Cofactor: Zn2+
Aktivators: Mg2+, Mn2+, Co2+
Inactivation: Complete inactivation after 5 minutes at +75°C.", "Name": "Properties" } ] } } ] }

Alkaline Phosphatase, recombinant, 20 U/μL

from bovine intestine, expressed in Pichia pastoris, solution

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