{ "ProductData": { "ID": "3.6.14.1.3.3", "ProductType": "Others", "BrandName": "Alkaline Phosphatase, recombinant, 20 U/μL", "ProductNameAddition": "from bovine intestine, expressed in Pichia pastoris, solution", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Alkaline Phosphatase recombinant 20 U uL" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-041-00", "StructureNodeStatus": "valid", "NodeName": "Phosphatases" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0136", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "04571363103", "MaterialDescription": "Alkaline Phosphatase rec.,MB Grade, 20 U", "RegisteredProductName": "Alkaline Phosphatase, recombinant, 20 U/uL", "GTIN": "07613336130711", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "20 U/μL", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Alkaline Phosphatase rec., MB Grade, 20 U\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "CB - Order Information", "Value": "Will be supplied as \"Alkaline Phosphatase rec., MB Grade, 20 U\". Unit of measure is \"kU\".
The enzyme is supplied without reaction buffer.", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Positioning", "Value": "Alkaline Phosphatase for 5' dephosphorylation of DNA and RNA.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Applications", "Value": "Use Alkalkine Phosphatase, recombinant for:

• Dephosphorylation of 5' phosphate from DNA and RNA
• Coupling to other proteins via its amino or carbohydrate groups, for detection in immunoassays and western blot analysis

", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Specification", "Value": "Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; MgCl₂, 1 mmol/L; ZnCl₂, 0.1 mmol/L; glycerol, 50% (v/v); pH approximately 7.6 at +4°C
Volume activity: ≥20 U/μL
Specific activity: ≥5 kU/mg protein
Unspecific endonucleases (λDNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 100 U after 1 hour incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "CAS Number: 9001-78-9
Enzyme activity: Alkaline Phosphatase, recombinant, 1 U/μL catalyzes the dephosphorylation of 5' phosphate from DNA and RNA.
pH activity optimum: 9.8
pH stability optimum: 8.0
Cofactor: Zn²⁺
Aktivators: Mg²⁺, Mn²⁺, Co²⁺
Inactivation: Complete inactivation after 5 minutes at +75°C.", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }