{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "Alkaline Phosphatase EIA Grade", "DisclaimerGroup1": "Non-IVD", "ControlTissue": [ "" ], "BrandName": "Alkaline Phosphatase, EIA Grade", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.7.3.1.1.1", "ProductNameAddition": "from calf intestine, solution", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Immunology Raw Materials", "NodeID": "02-0035" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Phosphatases", "NodeID": "70-041-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Enzymes", "NodeID": "02-0014" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "Labeling and Detection", "NodeID": "320-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10556602103", "MaterialDescription": "Phosphatase, Alkaline, Calf Intestine", "RegisteredProductName": "Alkaline Phosphatase, EIA Grade", "GTIN": "14038377155415", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''Phosphatase, Alkaline, Calf Intestine''. Unit of measure is ''g''." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Will be supplied as ''Phosphatase, Alkaline, Calf Intestine''. Unit of measure is ''g''.", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Alkaline phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, sugar alcohols, cyclic alcohols, phenols and amines. Phosphodiesters do not react with Alkaline Phosphatase, EIA Grade. The enzyme hydrolyzes PPi. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.", "Name": "Background Information" }, { "Language": "en", "Value": "Marker enzyme for preparing antibody- / antigen-enzyme conjugates incorporated in immunoassay reagents for colorimetric, fluorimetric and luminometric detection.", "Name": "Applications" }, { "Language": "en", "Value": "Appearance: Clear, colourless solution in NaCl, 3 mol/L; MgCl2, 1 mmol/L; ZnCl2, 0.1 mmol/L; Tea, 30 mmol/L
pH value: 7.0-8.0
Protein (A280, 1 mg/mL=1, against water): ≥10 mg/mL
Specific activity (+37°C, 4-NPP): ≥3,000 U/mg
Alkaline Phosphatase (HPLC): ≥95 area% (HPLC profile added to certificate)
Amino groups: 8-16 mol/mol
Carbohydrates, n=2: No limit
Molecular weight: ≥57,000 D
NH4: ≤30 ppm
pH 5.5 treatment (30 minutes): Corresponds to specification
Origin of bovine intestine: NZL
Stability: At +2 to +8°C within specification range for 15 months.", "Name": "Specification" }, { "Language": "en", "Value": "Nomenclature: Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
CAS Number: 9001-78-9
Molecular weight: ≥57 kD
Inhibitors: Pi, metal chelating agents, divalent heavy metal ions (e.g., Be2+, Zn2+), many amino acids (e.g., L-phenylalanine, L-tryptophan, L-cysteine), iodosobenzoate, iodoacet-amide.
Activators: Mg2+, Co 2+, Mn2+
pH optimum: 9.8
pH stability: 8.0
Thermal stability: Up to +40°C", "Name": "Properties" } ] } } ] }

Alkaline Phosphatase, EIA Grade

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