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Solubility: Clear, colorless solution in water (c = 50 mg/mL)
pH value: 6.5-8.0
Activity (+25°C, ethanol): ≥25 U/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase:≤0.01
Stability: At +2 to +8°C within specification range for 12 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "Nomenclature: Alcohol:NAD+ oxidoreductase
CAS Number: 9031-72-5
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10-2 mol/L
NAD: 7.4 x 10-5 mol/L
Acetaldehyde: 7.8 x 10-4 mol/L
NADH: 1.1 x 10-5 mol/L
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10-2 mol/L
NAD: 6.1 x 10-4 mol/L
Acetaldehyde: 6.7 x 10-4 mol/L
NADH: 1.8 x 10-5 mol/L
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate, iodoacetic acid, N-substituted maleinimides, Hg2+, Ag+ and Cu2+.
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine, cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H2O2 and aerial oxygen inactivate by oxidation of essential groups.
pH optimum: 9.0 (see figure)pH optimum
Temperature dependence: See figureTemperature dependence
pH stability: 6.0-8.0 (see figure)pH stability
Thermal stability: Up to +50°C (see figure)Thermal stability
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropanol and secondary butanol are slowly oxidized, while higher secondary and tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse reaction. The enzyme does not react with NADP.", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }

Alcohol Dehydrogenase, chemically modified

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