{ "ProductData": { "ID": "3.1.1.5.2.6", "ProductType": "Others", "BrandName": "Alcohol Dehydrogenase, chemically modified", "ProductNameAddition": "from yeast, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Alcohol Dehydrogenase chemically modified" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-014-00", "StructureNodeStatus": "valid", "NodeName": "Dehydrogenases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0011", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "01-00-00", "StructureNodeStatus": "valid", "NodeName": "Carbohydrate Testing" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11644980103", "MaterialDescription": "Alcohol Dehydrogenase, Yeast, Modified", "RegisteredProductName": "Alcohol Dehydrogenase, chemically modified", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Alcohol Dehydrogenase, Yeast, Modified\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "CB - Order Information", "Value": "Will be supplied as \"Alcohol Dehydrogenase, Yeast, Modified\". Unit of measure is \"MU\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Applications", "Value": "Use Alcohol Dehydrogenase in diagnostic tests for the determination of alcohol or aldehyde (formate).", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Positioning", "Value": "Dehydrogenase that catalyzes the interconversion of alcohols to the corresponding aldehydes.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Specification", "Value": "Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c = 50 mg/mL)
pH value: 6.5-8.0
Activity (+25°C, ethanol): ≥25 U/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase:≤0.01
Stability: At +2 to +8°C within specification range for 12 months.", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Properties", "Value": "Nomenclature: Alcohol:NAD⁺ oxidoreductase
CAS Number: 9031-72-5
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10^-2 mol/L
NAD: 7.4 x 10^-5 mol/L
Acetaldehyde: 7.8 x 10^-4 mol/L
NADH: 1.1 x 10^-5 mol/L
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10^-2 mol/L
NAD: 6.1 x 10^-4 mol/L
Acetaldehyde: 6.7 x 10^-4 mol/L
NADH: 1.8 x 10^-5 mol/L
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate, iodoacetic acid, N-substituted maleinimides, Hg²⁺, Ag⁺ and Cu²⁺.
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine, cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H₂O₂ and aerial oxygen inactivate by oxidation of essential groups.
pH optimum: 9.0 (see figure)pH optimum
Temperature dependence: See figureTemperature dependence
pH stability: 6.0-8.0 (see figure)pH stability
Thermal stability: Up to +50°C (see figure)Thermal stability
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropanol and secondary butanol are slowly oxidized, while higher secondary and tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse reaction. The enzyme does not react with NADP.", "Language": "en", "Country": "XG", "Code": "Properties" } ] } } ] }