{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "Alcohol Dehydrogenase", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "Alcohol Dehydrogenase", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.1.1.5.2.4", "ProductNameAddition": "from yeast, lyophilizate", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Nomenclature: Alcohol:NAD⁺ oxidoreductase
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10^-2 mol/L
NAD: 7.4 x 10^-5 mol/L
Acetaldehyde: 7.8 x 10^-4 mol/L
NADH: 1.1 x 10^-5 mol/L
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10^-2 mol/L
NAD: 6.1 x 10^-4 mol/L
Acetaldehyde: 6.7 x 10^-4 mol/L
NADH: 1.8 x 10^-5 mol/L
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate, iodoacetic acid, N-substituted maleinimides, Hg²⁺, Ag⁺ and Cu²⁺.
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine, cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H₂O₂ and aerial oxygen inactivate by oxidation of essential groups.
pH optimum: 9.0 (see figure)
Temperature dependence: See figure
pH stability: 6.0-8.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropanol and secondary butanol are slowly oxidized, while higher secondary and tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse reaction. The enzyme does not react with NADP.
Remarks:Alcohol dehydrogenase tends to show turbidity in solution at +37°C storage. Modified Alcohol dehydrogenase shows no turbidity for at least 4 weeks in solution at +37°C.", "Name": "CB - Properties" }, { "Language": "English", "Value": "Appearance: White lyophilizate (50 mg lyophilizate contain approximately 30 mg enzyme protein,15 mg sucrose, 5 mg phosphate)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, ethanol): No limit
Specific activity: ≥400 U/mg (protein)
Protein (Biuret): ≥0.5 mg/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry.", "Name": "CB - Specification" }, { "Language": "English", "Value": "Use Alcohol Dehydrogenase in diagnostic tests for the determination of alcohol or aldehyde (formate).", "Name": "Applications" }, { "Language": "English", "Value": "Will be supplied as \"Alcohol Dehydrogenase, Yeast\". Unit of measure is \"MU\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Dehydrogenase that catalyzes the interconversion of alcohols to the corresponding aldehydes.", "Name": "Positioning" }, { "Language": "English", "Value": "Nomenclature: Alcohol:NAD⁺ oxidoreductase
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10^-2 mol/L
NAD: 7.4 x 10^-5 mol/L
Acetaldehyde: 7.8 x 10^-4 mol/L
NADH: 1.1 x 10^-5 mol/L
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10^-2 mol/L
NAD: 6.1 x 10^-4 mol/L
Acetaldehyde: 6.7 x 10^-4 mol/L
NADH: 1.8 x 10^-5 mol/L
Inhibitors:
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate, iodoacetic acid, N-substituted maleinimides, Hg²⁺, Ag⁺ and Cu²⁺.
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine, cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H₂O₂ and aerial oxygen inactivate by oxidation of essential groups.
pH optimum: 9.0 (see figure)
Temperature dependence: See figure
pH stability: 6.0-8.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropanol and secondary butanol are slowly oxidized, while higher secondary and tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse reaction. The enzyme does not react with NADP.
Remarks:Alcohol dehydrogenase tends to show turbidity in solution at +37°C storage. Modified Alcohol dehydrogenase shows no turbidity for at least 4 weeks in solution at +37°C.", "Name": "Properties" }, { "Language": "English", "Value": "Appearance: White lyophilizate (50 mg lyophilizate contain approximately 30 mg enzyme protein,15 mg sucrose, 5 mg phosphate)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, ethanol): No limit
Specific activity: ≥400 U/mg (protein)
Protein (Biuret): ≥0.5 mg/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Enzymes", "StructureSystemID": null, "NodeID": "02-0014" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->Dehydrogenases", "StructureSystemID": null, "NodeID": "70-014-00" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "Applications", "NodeName": "Carbohydrate Testing", "StructureSystemID": null, "NodeID": "15-00-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Clinical Chemistry Raw Materials", "StructureSystemID": null, "NodeID": "02-0011" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11452541103", "MaterialDescription": "Alcohol Dehydrogenase, Yeast", "RegisteredProductName": "", "GTIN": "14038377155477", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Alcohol Dehydrogenase, Yeast\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }