{ "ProductData": { "ID": "", "ProductType": "Others", "BrandName": "Alcohol Dehydrogenase", "ProductNameAddition": "from yeast, lyophilizate", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "Alcohol Dehydrogenase" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE_.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0014", "StructureNodeStatus": "valid", "NodeName": "Enzymes" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeID": "70-014-00", "StructureNodeStatus": "valid", "NodeName": "Dehydrogenases" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeID": "01-01-01-07-00", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeID": "02-0011", "StructureNodeStatus": "valid", "NodeName": "Clinical Chemistry Raw Materials" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeID": "01-00-00", "StructureNodeStatus": "valid", "NodeName": "Carbohydrate Testing" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeID": "520", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeID": "180-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeID": "999-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeID": "999", "StructureNodeStatus": "invalid", "NodeName": "" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeID": "99-00-00", "StructureNodeStatus": "invalid", "NodeName": "" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "11452541103", "MaterialDescription": "Alcohol Dehydrogenase, Yeast", "RegisteredProductName": "Alcohol Dehydrogenase", "GTIN": "14038377155477", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "custom fill", "PackSizeDescPIM360": "Not Available", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"Alcohol Dehydrogenase, Yeast\". Unit of measure is \"MU\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Name": "Applications", "Value": "Use Alcohol Dehydrogenase in diagnostic tests for the determination of alcohol or aldehyde (formate).", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "CB - Order Information", "Value": "Will be supplied as \"Alcohol Dehydrogenase, Yeast\". Unit of measure is \"MU\".", "Language": "en", "Country": "XG", "Code": "CB - Order Information" }, { "Name": "Positioning", "Value": "Dehydrogenase that catalyzes the interconversion of alcohols to the corresponding aldehydes.", "Language": "en", "Country": "XG", "Code": "Positioning" }, { "Name": "Properties", "Value": "Nomenclature: Alcohol:NAD+ oxidoreductase
CAS Number: 9031-72-5
Molecular weight: 141 kD (pH 7.0)
Isoelectric point: 5.4-5.8
Michaelis constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 1.3 x 10-2 mol/L
NAD: 7.4 x 10-5 mol/L
Acetaldehyde: 7.8 x 10-4 mol/L
NADH: 1.1 x 10-5 mol/L
Inhibitor constants (Phosphate buffer, pH 7.15, +25°C):
Ethanol: 4.3 x 10-2 mol/L
NAD: 6.1 x 10-4 mol/L
Acetaldehyde: 6.7 x 10-4 mol/L
NADH: 1.8 x 10-5 mol/L
-SH-reagents and heavy metals, such as derivatives, 4-chloromercuribenzoate, iodoacetic acid, N-substituted maleinimides, Hg2+, Ag+ and Cu2+.
-Complexing agents, e.g., o- phenanthroline, EDTA, oxalate.
-NAD analogs and NAD partial structures, e.g., NADP, NADH, ADP, ADP-ribose.
-Substances, which react with enzyme bound NAD, e.g., sulfite, hydroxylamine, cyanide.
-Substrate analogs, e.g., fluoroethanol.
-Oxidizers, e.g., H2O2 and aerial oxygen inactivate by oxidation of essential groups.
pH optimum: 9.0 (see figure)pH optimum
Temperature dependence: See figureTemperature dependence
pH stability: 6.0-8.0 (see figure)pH stability
Thermal stability: Up to +50°C (see figure)Thermal stability
Specificity: Alcohol dehydrogenase oxidizes primary alcohols. Isopropanol and secondary butanol are slowly oxidized, while higher secondary and tertiary alcohols do not react. Numerous aldehydes are reduced in the reverse reaction. The enzyme does not react with NADP.
Remarks:Alcohol dehydrogenase tends to show turbidity in solution at +37°C storage. Modified Alcohol dehydrogenase shows no turbidity for at least 4 weeks in solution at +37°C.", "Language": "en", "Country": "XG", "Code": "Properties" }, { "Name": "Specification", "Value": "Appearance: White lyophilizate (50 mg lyophilizate contain approximately 30 mg enzyme protein,15 mg sucrose, 5 mg phosphate)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, ethanol): No limit
Specific activity: ≥400 U/mg (protein)
Protein (Biuret): ≥0.5 mg/mg lyophilizate
Contaminants (expressed as percentage of Alcohol Dehydrogenase activity):
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry.", "Language": "en", "Country": "XG", "Code": "Specification" } ] } } ] }

Alcohol Dehydrogenase

from yeast, lyophilizate


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