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Vial	Content	Composition
AQB	Reagent cassette, 200 tests	Reagent 1 (R1): Amino acid arylamidase, 2.1 kU/L, glutamate oxidase, 200 U/L; catalase, 600 kU/L; 4-aminoantipyrine (AAP), 0.4 mmol/L; TOOS, 2 mmol/L; preservative; HEPES buffer, pH 7.0.
Start Reagent (SR): Glutaminase, 2.5 kU/L; peroxidase, 8.6 kU/L; TOOS, 2 mmol/L; preservative; HEPES buffer, pH 7.0.

", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Additional Equipment and Reagent Required", "Value": "

• Cedex Bio HT Analyzer*, including accessories and disposables
• Standard laboratory equipment

• Calibrator D Bio*
• Control D Level 1 Bio*
• Control D Level 2 Bio*
• Control D Level 3 Bio*

Optional, for correction of free Glutamine background:

• Glutamine V2 Bio HT* 
• Calibrator B Bio* 
• Control B Level 1 Bio* 
• Control B Level 2 Bio* 
• Control B Level 3 Bio* 

", "Language": "en", "Country": "XG", "Code": "Additional Equipment and Reagent Required" }, { "Name": "Applications", "Value": "Glutamine is an essential nutrient required in cell cultures and microbial fermentation. Under culture conditions, glutamine has the disadvantage of fast degradation, producing cell-toxic ammonia. The dipeptide Ala-Gln is heat-stable and shows no spontaneous breakdown. Living cells in a culture are able to gradually release glutamine from the Ala-Gln dipeptide by secretion of peptidases. Depending on the number of cells and on the intensity of their metabolism, more dipeptide has to be fed over the culture time to achieve permanently an optimal supply. For control of culture media and for monitoring in a culture process, the Ala-Gln concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Language": "en", "Country": "XG", "Code": "Applications" }, { "Name": "Specification", "Value": "Method: Enzymatic photometric assay using hydrolysis by peptidase and deamination of glutamine by glutaminase.
Recovery: ±15%", "Language": "en", "Country": "XG", "Code": "Specification" }, { "Name": "Safety Information", "Value": "Precautions

• Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
• Do not eat, drink, or smoke in the laboratory work area.
• Do not pipette by mouth.
• Wear protective disposable gloves, laboratory coats, and eye pro\u00adtection, when handling samples and kit reagents.
• Wash hands thoroughly after handling samples and reagents.

Waste handling

• Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
• Safety Data Sheets (SDS) are available online at dialog.roche.com, or upon request from the local Roche office.

", "Language": "en", "Country": "XG", "Code": "Safety Information" } ] } } ] }