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"Health_Topics", "NameofStructureSystem": "Health Topics", "StructureNodeID": "99-00-00", "StructureGroupPath": "NOT APPLICABLE", "StructureGroupName": "NOT APPLICABLE" } ] } ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Onboard stability: After first use on the instrument, the reagent cas- sette is stable for 12 weeks.", "Name": "Storage Conditions (Working Solution)" }, { "Language": "en", "Value": "Store the reagent cassette at +2 to +8°C.", "Name": "Storage Conditions (Product)" }, { "Language": "en", "Value": "Unit of measure is \"piece\".", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Measuring Range
Ala-Gln concentration can be determined in the following ranges:
  • AQB: 21 to 2,172 mg/L (0.1 to 10 mmol/L)
  • AQD: 217 to 21,720 mg/L (1.0 to 100 mmol/L),
up to maximal solubility with automatic predilution.

Conversion Factors
Conversion factors for Ala-Gln concentration:
•1 mmol/L = 217.22 mg/L
•1 g/L = 4.604 mmol/L

Traceability
This method has been standardized against Ala-Gln net weight.

Precision
Representative performance data on Cedex Bio HT Analyzers are shown. Results obtained in individual laboratories may differ.
Precision was determined in samples of three concentration levels. Coefficients of variation (CV) were calculated for in-run precision (n=21) and inter-run precision (on 10 days).

  Level 1 Level 2 Level 3
Mean 0.5 mmol/L
(109 mg/L)
4.0 mmol/L
(869 mg/L)
7.0 mmol/L
(1520 mg/L)
CV in-run 0.7% 0.4% 0.5%
CV inter-run 1.7% 1.4% 1.5%
", "Name": "Background Information - Help Corner" }, { "Language": "en", "Value": "
VialContentComposition
AQBReagent cassette,
200 tests
Reagent 1 (R1): Amino acid arylamidase, 2.1 kU/L, glutamate oxidase, 200 U/L; catalase, 600 kU/L; 4-aminoantipyrine (AAP), 0.4 mmol/L; TOOS, 2 mmol/L; preservative; HEPES buffer, pH 7.0.
Start Reagent (SR): Glutaminase, 2.5 kU/L; peroxidase, 8.6 kU/L; TOOS, 2 mmol/L; preservative; HEPES buffer, pH 7.0.
", "Name": "Content" }, { "Language": "en", "Value": "Cedex Bio HT test definition:
Measuring mode           Absorbance
Abs. calculation mode     Endpoint
Reaction mode                 R1-S-SR
Reaction direction            Increase
Wavelength A/B                 552 nm
Calc. first/last                    82/114
Unit                                     mmol/L, mg/L

Pipetting parameters:
R1                      140 μL
Sample                 4 μL
SR                        42 μL
Total volume    186 μL

Calibration:
CalibratorCalibrator D Bio    (CAL D, 07-1141-1)
Calibration mode                    Linear regression
Calibration interval                 84 days,
              and if recalibration is required due to QC results.

Quality control:
Controls            
Control D Level 1 Bio (COND1, 07-1143-8)
Control D Level 2 Bio (COND2, 07-1144-6)
Control D Level 3 Bio (COND3, 07-1145-4)

Use the recommended control material. Other suitable control material can also be used.
Intervals / limits       Control intervals and acceptance limits should be adapted to each laboratory's individual requirements.
If values do not fall within the defined limits, corrective measures and recalibration are required.", "Name": "Protocols - Help Corner" }, { "Language": "en", "Value": "This product is intended for quantitative determination of the cell culture supplement alanyl-glutamine (GlutaMAX) in aqueous solutions using the Cedex Bio HT Analyzer.", "Name": "Product Description" }, { "Language": "en", "Value": "
Analyzer and accessories
  • Cedex Bio HT Analyzer*, including accessories and disposables
  • Standard laboratory equipment
Calibrators and Controls
  • Calibrator D Bio*
  • Control D Level 1 Bio*
  • Control D Level 2 Bio*
  • Control D Level 3 Bio*
  • Glutamine V2 Bio HT* (only for correction of free glutamine background)
  • Calibrator B Bio* (only for correction of free glutamine background)
  • Control B Level 1 Bio* (only for correction of free glutamine background)
  • Control B Level 2 Bio* (only for correction of free glutamine background)
  • Control B Level 3 Bio* (only for correction of free glutamine background)
", "Name": "Additional Equipment and Reagent Required" }, { "Language": "en", "Value": "Glutamine is an essential nutrient required in cell cultures and microbial fermentation. Under culture conditions, glutamine has the disadvantage of fast degradation, producing cell-toxic ammonia. The dipeptide Ala-Gln is heat-stable and shows no spontaneous breakdown. Living cells in a culture are able to gradually release glutamine from the Ala-Gln dipeptide by secretion of peptidases. Depending on the number of cells and on the intensity of their metabolism, more dipeptide has to be fed over the culture time to achieve permanently an optimal supply. For control of culture media and for monitoring in a culture process, the Ala-Gln concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.", "Name": "Applications" }, { "Language": "en", "Value": "Method: Enzymatic photometric assay using hydrolysis by peptidase and deamination of glutamine by glutaminase.
Recovery: ±15%", "Name": "Specification" }, { "Language": "en", "Value": "Precautions
  • Handle all samples as if potentially infectious, using safe laboratory procedures. As the sensitivity and titer of potential pathogens in the sample material can vary, the operator must optimize pathogen inactivation and follow the appropriate measures according to local safety regulations.
  • Do not eat, drink, or smoke in the laboratory work area.
  • Do not pipette by mouth.
  • Wear protective disposable gloves, laboratory coats, and eye pro\u00adtection, when handling samples and kit reagents.
  • Wash hands thoroughly after handling samples and reagents.
Waste handling
  • Discard unused reagents and waste in accordance with country, federal, state, and local regulations.
  • Safety Data Sheets (SDS) are available online at dialog.roche.com, or upon request from the local Roche office.
", "Name": "Safety Information" } ] } } ] }

Ala-Gln Bio HT

Test kit for the Cedex Bio HT Analyzer

cedex-ala-gln-bio-ht

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