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  • Optimize your SNP analysis.
    Discriminate paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
  • Simplify PCR set-up.
    Pipett and handle the hot start reaction mix at ambient temperature.
  • Simplify assay design.
    Create capable PCR assays with a minimum of optimization efforts, also multiplex PCR applications.
", "Name": "Benefits" }, { "Language": "en", "Value": "Use ActiTaq exo DNA Polymerase for:
  • SNP analysis and genotyping
  • Allele-specific PCR
  • Multiplexing up to 350 bp
  • Random primed PCR
", "Name": "Applications" }, { "Language": "en", "Value": "The 5x concentrated master mix contains a chemically modified truncated Taq DNA Polymerase and all reagents (except primers, probes, and template) needed for real-time DNA detection assays with various probe formats except for hydrolysis probes due to the lack of 5'-3' exonuclease activity.", "Name": "Product Description" }, { "Language": "en", "Value": "Hot start DNA master mix containing a 5'-3' exonuclease deficient hot start Taq DNA polymerase for optimal detection of mismatches.", "Name": "Positioning" }, { "Language": "en", "Value": "The master mix can be stored in the refrigerator (+2 to +8°C) for at least 4 week without loss of activity and performance. The complete PCR mix (Master + primers + probes + template) is stable for up to 24 hours at +15 to +25°C.", "Name": "Properties" }, { "Language": "en", "Value": "Appearance: Clear colorless solution
Performance test in qPCR (amplification with ApoB fragment): Corresponds to specification
Sensitivity (≤100 copies; negative control ≥40 cycles): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" } ] } } ] }

ActiTaq exo Genotyping Master

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