{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "DisclaimerGroup2": null, "ProductNameGlobal": "ActiTaq exo Genotyping Master", "DisclaimerGroup1": "Other - Non IVD", "ControlTissue": [ "" ], "BrandName": "ActiTaq exo Genotyping Master", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.5.8.1.1.3", "ProductNameAddition": "5x concentrated", "Species": [ "" ], "Clone": "" }, "ProductSpec": { "ProductSpecVariant": { "Chapters": [ { "Language": "English", "Value": "Will be supplied as \"ActiTaq delta exo Genotyping Master\". Unit of measure is \"mL\".", "Name": "CB - Order Information" }, { "Language": "English", "Value": "Appearance: Clear colorless solution
Performance test in qPCR (amplification with ApoB fragment): Corresponds to specification
Sensitivity (≤100 copies; negative control ≥40 cycles): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "CB - Specification" }, { "Language": "English", "Value": "The master mix can be stored in the refrigerator (+2 to +8°C) for at least 4 week without loss of activity and performance. The complete PCR mix (Master + primers + probes + template) is stable for up to 24 hours at +15 to +25°C.", "Name": "CB - Properties" }, { "Language": "English", "Value": "

• Optimize your SNP analysis.
  Discriminate paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
• Simplify PCR set-up.
  Pipett and handle the hot start reaction mix at ambient temperature.
• Simplify assay design.
  Create capable PCR assays with a minimum of optimization efforts, also multiplex PCR applications.

", "Name": "Benefits" }, { "Language": "English", "Value": "Use ActiTaq exo DNA Polymerase for:

• SNP analysis and genotyping
• Allele-specific PCR
• Multiplexing up to 350 bp
• Random primed PCR

", "Name": "Applications" }, { "Language": "English", "Value": "The 5x concentrated master mix contains a chemically modified truncated Taq DNA Polymerase and all reagents (except primers, probes, and template) needed for real-time DNA detection assays with various probe formats except for hydrolysis probes due to the lack of 5'-3' exonuclease activity.", "Name": "Product Description" }, { "Language": "English", "Value": "Hot start DNA master mix containing a 5'-3' exonuclease deficient hot start Taq DNA polymerase for optimal detection of mismatches.", "Name": "Positioning" }, { "Language": "English", "Value": "The master mix can be stored in the refrigerator (+2 to +8°C) for at least 4 week without loss of activity and performance. The complete PCR mix (Master + primers + probes + template) is stable for up to 24 hours at +15 to +25°C.", "Name": "Properties" }, { "Language": "English", "Value": "Appearance: Clear colorless solution
Performance test in qPCR (amplification with ApoB fragment): Corresponds to specification
Sensitivity (≤100 copies; negative control ≥40 cycles): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.", "Name": "Specification" } ] } }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemName": "OWP Product Solutions", "NodeName": "CustomBiotech", "StructureSystemID": null, "NodeID": "520" }, { "StructureSystemName": "OWP Product Types", "NodeName": "Raw Materials and Reagents->DNA Master Mixes", "StructureSystemID": null, "NodeID": "70-012-00" }, { "StructureSystemName": "Product Grouping", "NodeName": "CustomBiotech->Molecular Raw Materials", "StructureSystemID": null, "NodeID": "02-0136" }, { "StructureSystemName": "OWP Organization", "NodeName": "Roche Diagnostics Solution (RDS)->Customer Areas->Core lab->CustomBiotech and LifeScience", "StructureSystemID": null, "NodeID": "01-01-01-07-00" }, { "StructureSystemName": "OWP Techniques", "NodeName": "PCR/qPCR", "StructureSystemID": null, "NodeID": "210-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "06362281001", "MaterialDescription": "ActiTaq delta exo Genotyping Master", "RegisteredProductName": "", "GTIN": "", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "50 mL", "PackSizeDescPIM360": "", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as \"ActiTaq delta exo Genotyping Master\". Unit of measure is \"mL\"." } ] }, "Product2Products": { "Product2ProductReference": [ ] } }