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"Language": "en",
"Value": "Use 7-Deaza-dGTP as a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.",
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"Value": "Comparison of 7-Deaza-dGTP with dGTP and dITP showed that 7-Deaza-dGTP gives enhanced resolution compared with dGTP, resulting increased readability over long sequence regions compared with dITP. For sequencing reactions, dGTP is replaced by the same amount of 7-Deaza-dGTP in all four dideoxy-NTP solutions.
7-Deaza-dGTP performs equally well to all the other types of dideoxy sequencing and polymerization techniques. Partial substitution of 7-Deaza-dGTP for dGTP in PCR can improve the yield of reaction products for GC-rich templates containing strong secondary structures. Elimination of spurious GC-hydrogen bonding and relaxation of the secondary structure results in more efficient and specific PCR-product synthesis.
Incorporation of 7-Deaza-dGTP into DNA alters the fluorescent staining and electrophoretic mobility of the DNA.",
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"Value": "Use 7-Deaza-dGTP instead of dGTP in primer-extension reactions or PCR and receive a better resolution of GC-rich regions.",
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Appearance: Clear, colorless solution
pH value: 6.8-7.2
7-Deaza-2'-dGTP (1 μmol ≙ 13.4 A
259 units): 10.0-11.0 mmol/L
7-Deaza-2'-dGTP (HPLC): ≥95 area%
7-Deaza-2'-dGDP (HPLC): ≤4 area%
A250/A260: 0.84±0.04
A280/A260: 0.65±0.03
A290/A260: 0.53±0.03
Stability: At -15 to -25°C within specification range for 30 months.",
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Nomenclature: 7-Deaza-2'-deoxy-guanosine-5'-triphosphate
CAS Number: 101515-08-6
Formula: C
11H
17N
4O
13P
3Molecular weight: 506.2 D",
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