{ "ProductData": { "RelatedLinks": "", "RegulatoryDisclaimer1": "For further processing only.", "RegulatoryDisclaimer2": "", "StainLocalization": [ "" ], "ProductType": "Others", "DisclaimerGroup2": null, "ProductNameGlobal": "7-Deaza-2 -dGTP", "DisclaimerGroup1": "", "ControlTissue": [ "" ], "BrandName": "7-Deaza-2'-dGTP", "LicenseDisclaimers": [ ], "Isotypes": "", "RegulatoryDisclaimer3": "", "ID": "3.6.5.3.14.1", "ProductNameAddition": "lithium salt, 10 mM", "Species": [ "" ], "Clone": "" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxomony": { "Product2TaxomonyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Nucleotides", "NodeID": "02-0018" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Deoxyucleotides", "NodeID": "70-015-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "RT-PCR/qRT-PCR", "NodeID": "300-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Nucleotides", "NodeID": "02-0018" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "NodeName": "Deoxyucleotides", "NodeID": "70-015-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "NodeName": "RT-PCR/qRT-PCR", "NodeID": "300-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "NodeName": "Manufacturing", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "NodeName": "NOT APPLICABLE", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "NodeName": "NOT APPLICABLE", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10987891103", "MaterialDescription": "7-Deaza-2'-deoxy-GTP, Di-Li", "RegisteredProductName": "7-Deaza-2'-dGTP", "GTIN": "14038377155446", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "100 mL", "PackSizeDescPIM360": "1,000 µmol", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''7-Deaza-2'-deoxy-GTP, Di-Li''. Unit of measure is ''μmol''." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use 7-Deaza-dGTP as a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Comparison of 7-Deaza-dGTP with dGTP and dITP showed that 7-Deaza-dGTP gives enhanced resolution compared with dGTP, resulting increased readability over long sequence regions compared with dITP. For sequencing reactions, dGTP is replaced by the same amount of 7-Deaza-dGTP in all four dideoxy-NTP solutions.
7-Deaza-dGTP performs equally well to all the other types of dideoxy sequencing and polymerization techniques. Partial substitution of 7-Deaza-dGTP for dGTP in PCR can improve the yield of reaction products for GC-rich templates containing strong secondary structures. Elimination of spurious GC-hydrogen bonding and relaxation of the secondary structure results in more efficient and specific PCR-product synthesis.
Incorporation of 7-Deaza-dGTP into DNA alters the fluorescent staining and electrophoretic mobility of the DNA.", "Country": "XG", "Code": "Background Information", "Name": "Background Information" }, { "Language": "en", "Value": "Will be supplied as ''7-Deaza-2'-deoxy-GTP, Di-Li''. Unit of measure is ''μmol''.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use 7-Deaza-dGTP instead of dGTP in primer-extension reactions or PCR and receive a better resolution of GC-rich regions.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
pH value: 6.8-7.2
7-Deaza-2'-dGTP (1 μmol ≙ 13.4 A259 units): 10.0-11.0 mmol/L
7-Deaza-2'-dGTP (HPLC): ≥95 area%
7-Deaza-2'-dGDP (HPLC): ≤4 area%
A250/A260: 0.84±0.04
A280/A260: 0.65±0.03
A290/A260: 0.53±0.03
Stability: At -15 to -25°C within specification range for 30 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "Nomenclature: 7-Deaza-2'-deoxy-guanosine-5'-triphosphate
CAS Number: 101515-08-6
Formula: C11H17N4O13P3
Molecular weight: 506.2 D", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }

7-Deaza-2'-dGTP

lithium salt, 10 mM

logo

Ordering Information

Technical documents

for certificates, method sheets (instructions for use), safety data sheets and software downloads
error errorMessage
Sorry, we couldn't find the content you are looking for
Please try again later