{ "ProductData": { "ID": "3.6.5.3.14.1", "ProductType": "Others", "BrandName": "7-Deaza-2'-dGTP", "ProductNameAddition": "lithium salt, 10 mM", "RegulatoryDisclaimer1": "For further processing only.", "DisclaimerGroup1": "", "RegulatoryDisclaimer2": null, "DisclaimerGroup2": null, "RegulatoryDisclaimer3": null, "SampleType": [ ], "LicenseDisclaimers": [ ], "RelatedLinks": "", "Clone": "", "ControlTissue": [ "" ], "ISOtypes": "", "Species": [ "" ], "StainLocalization": [ "" ], "ProductNameGlobal": "7-Deaza-2 -dGTP" }, "ProductImageDetails": { "ImagePath": "https://pim-media.roche.com/Images/CB_IMAGE.png", "ImageType": "Image main" }, "Product2Taxonomy": { "Product2TaxonomyReferences": [ { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Nucleotides", "NodeID": "02-0018" }, { "StructureSystemIdentifier": "OWP_Product_Types", "StructureSystemName": "Product Types", "StructureNodeStatus": "valid", "NodeName": "Deoxyucleotides", "NodeID": "70-015-00" }, { "StructureSystemIdentifier": "OWP_Organization", "StructureSystemName": "Organization", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech and LifeScience", "NodeID": "01-01-01-07-00" }, { "StructureSystemIdentifier": "Product_Grouping", "StructureSystemName": "Product Grouping", "StructureNodeStatus": "valid", "NodeName": "Molecular Raw Materials", "NodeID": "02-0136" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "valid", "NodeName": "PCR/qPCR", "NodeID": "210-00" }, { "StructureSystemIdentifier": "OWP_Techniques", "StructureSystemName": "Techniques", "StructureNodeStatus": "valid", "NodeName": "RT-PCR/qRT-PCR", "NodeID": "300-00" }, { "StructureSystemIdentifier": "Product_Solutions", "StructureSystemName": "Product Solutions", "StructureNodeStatus": "valid", "NodeName": "CustomBiotech", "NodeID": "520" }, { "StructureSystemIdentifier": "Lab_Type", "StructureSystemName": "Lab Types", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "180-00" }, { "StructureSystemIdentifier": "Applications", "StructureSystemName": "Applications", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Disease_Areas", "StructureSystemName": "Disease Areas", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "Pathogens", "StructureSystemName": "Pathogens", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" }, { "StructureSystemIdentifier": "OWP_Family", "StructureSystemName": "Product Families", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "999" }, { "StructureSystemIdentifier": "Health_Topics", "StructureSystemName": "Health Topics", "StructureNodeStatus": "invalid", "NodeName": "", "NodeID": "99-00-00" } ] }, "Product2Materials": { "P2MaterialReferences": [ { "MaterialNum": "10987891103", "MaterialDescription": "7-Deaza-2'-deoxy-GTP, Di-Li", "RegisteredProductName": "7-Deaza-2'-dGTP", "GTIN": "14038377155446", "ProductCategoryText": "Reagents, kits", "OldMaterialNumber": "", "PackSizePIM360": "100 mL", "PackSizeDescPIM360": "1,000 µmol", "MaterialAnnotation": "CustomBiotech product. Please contact your local representative.", "ReadyForUse": "", "OrderInformation": "Will be supplied as ''7-Deaza-2'-deoxy-GTP, Di-Li''. Unit of measure is ''μmol''." } ] }, "Product2Products": { "Product2ProductReference": [ ] }, "ProductSpec": [ { "ProductSpecVariant": { "Chapters": [ { "Language": "en", "Value": "Use 7-Deaza-dGTP as a substrate for most DNA polymerases, including Taq DNA polymerase.
7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Comparison of 7-Deaza-dGTP with dGTP and dITP showed that 7-Deaza-dGTP gives enhanced resolution compared with dGTP, resulting increased readability over long sequence regions compared with dITP. For sequencing reactions, dGTP is replaced by the same amount of 7-Deaza-dGTP in all four dideoxy-NTP solutions.
7-Deaza-dGTP performs equally well to all the other types of dideoxy sequencing and polymerization techniques. Partial substitution of 7-Deaza-dGTP for dGTP in PCR can improve the yield of reaction products for GC-rich templates containing strong secondary structures. Elimination of spurious GC-hydrogen bonding and relaxation of the secondary structure results in more efficient and specific PCR-product synthesis.
Incorporation of 7-Deaza-dGTP into DNA alters the fluorescent staining and electrophoretic mobility of the DNA.", "Country": "XG", "Code": "Background Information", "Name": "Background Information" }, { "Language": "en", "Value": "Will be supplied as ''7-Deaza-2'-deoxy-GTP, Di-Li''. Unit of measure is ''μmol''.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use 7-Deaza-dGTP instead of dGTP in primer-extension reactions or PCR and receive a better resolution of GC-rich regions.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
pH value: 6.8-7.2
7-Deaza-2'-dGTP (1 μmol ≙ 13.4 A₂₅₉ units): 10.0-11.0 mmol/L
7-Deaza-2'-dGTP (HPLC): ≥95 area%
7-Deaza-2'-dGDP (HPLC): ≤4 area%
A₂₅₀/A₂₆₀: 0.84±0.04
A₂₈₀/A₂₆₀: 0.65±0.03
A₂₉₀/A₂₆₀: 0.53±0.03
Stability: At -15 to -25°C within specification range for 30 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "Nomenclature: 7-Deaza-2'-deoxy-guanosine-5'-triphosphate
CAS Number: 101515-08-6
Formula: C₁₁H₁₇N₄O₁₃P₃
Molecular weight: 506.2 D", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }