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7-Deaza-dGTP is used in the dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA.", "Country": "XG", "Code": "Applications", "Name": "Applications" }, { "Language": "en", "Value": "Comparison of 7-Deaza-dGTP with dGTP and dITP showed that 7-Deaza-dGTP gives enhanced resolution compared with dGTP, resulting increased readability over long sequence regions compared with dITP. For sequencing reactions, dGTP is replaced by the same amount of 7-Deaza-dGTP in all four dideoxy-NTP solutions.
7-Deaza-dGTP performs equally well to all the other types of dideoxy sequencing and polymerization techniques. Partial substitution of 7-Deaza-dGTP for dGTP in PCR can improve the yield of reaction products for GC-rich templates containing strong secondary structures. Elimination of spurious GC-hydrogen bonding and relaxation of the secondary structure results in more efficient and specific PCR-product synthesis.
Incorporation of 7-Deaza-dGTP into DNA alters the fluorescent staining and electrophoretic mobility of the DNA.", "Country": "XG", "Code": "Background Information", "Name": "Background Information" }, { "Language": "en", "Value": "Will be supplied as ''7-Deaza-2'-deoxy-GTP, Di-Li''. Unit of measure is ''μmol''.", "Country": "XG", "Code": "CB - Order Information", "Name": "CB - Order Information" }, { "Language": "en", "Value": "Use 7-Deaza-dGTP instead of dGTP in primer-extension reactions or PCR and receive a better resolution of GC-rich regions.", "Country": "XG", "Code": "Positioning", "Name": "Positioning" }, { "Language": "en", "Value": "Appearance: Clear, colorless solution
pH value: 6.8-7.2
7-Deaza-2'-dGTP (1 μmol ≙ 13.4 A259 units): 10.0-11.0 mmol/L
7-Deaza-2'-dGTP (HPLC): ≥95 area%
7-Deaza-2'-dGDP (HPLC): ≤4 area%
A250/A260: 0.84±0.04
A280/A260: 0.65±0.03
A290/A260: 0.53±0.03
Stability: At -15 to -25°C within specification range for 30 months.", "Country": "XG", "Code": "Specification", "Name": "Specification" }, { "Language": "en", "Value": "Nomenclature: 7-Deaza-2'-deoxy-guanosine-5'-triphosphate
CAS Number: 101515-08-6
Formula: C11H17N4O13P3
Molecular weight: 506.2 D", "Country": "XG", "Code": "Properties", "Name": "Properties" } ] } } ] }

7-Deaza-2'-dGTP

lithium salt, 10 mM

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