Shorter turnaround times in molecular PoC with the right raw materials

The value of PoC testing lies in delivering on-the-spot results for treatment decisions.

Turnaround can be shortened by boosting assay speed, especially in the case of RT-PCR and PCR-based tests.

KAPA3G, designed for speed and robustness, can perform a full PCR within only 23 minutes (all polymerases were used according to manufacturer's instructions, data on file at Roche).

The protocols may become even longer in the case of RT-PCR because of the additional reverse transcription step. Fortunately, NxtScript 2G does it very fast and completes reverse transcription in only 5 minutes. And what’s more, NxtScript 2G is a heat-powered reverse transcriptase evolved toward high thermostablity. It performs reverse transcription at high temperatures (up to 70°C) to easily tackle any difficult RNA target.

Robust to variation and inhibitors

Sample preparation is often time-consuming and variable. Thus, developers of PoC diagnostics seek to simplify – if not completely eliminate – sample processing. As a result, analyses may be exposed to PCR-inhibiting substances naturally contained in biological matrices. Roche CustomBiotech enzymes, such as KAPA3G, have been tested against a broad range of inhibitors inherent to liquid biopsies, tissues, and standard sample preparation methods, and have demonstrated robust performance even with low-processed samples.