In vitro synthesis of mRNA molecules is a widely used laboratory procedure that is central to RNA research and for the development of therapeutic applications. Although already decades in development, mRNA was first validated as new drug class with the approval of mRNA COVID-19 vaccines. mRNA is now being considered for diverse applications like protein replacement, cell reprogramming, and gene editing. Individual constructs and intended uses require a tailored manufacturing process. Moving forward, expanded tool-box to produce mRNA will be needed.
In response to customer requests, we now added SP6 RNA Polymerase in a GMP Grade and fit-for-purpose format to our portfolio. This expands options in mRNA manufacturing for our customers. Here we present some starting guidelines for the use of SP6 RNA Polymerase.