Immunoassay Interference Blocker

In a patient sample they can interact with analyte-specific antibodies from an assay system. These interactions decrease measurement accuracy and can produce false-positive or false-negative results. Most diagnostic immunoassay reagents use mouse monoclonal antibodies, so human anti-mouse antibodies (HAMA) are the most common cause of interferences.

Up to 10% of test samples contain HAMA antibodies, and their characteristics vary greatly with respect to isotype, specificity, and concentration range. Our immunoassay interference blocker toolbox provides effective and highly specific HAMA interference blocking solutions that account for type of interaction and specific assay design. Plus, our HAMA-interference controls enable evaluation of assay accuracy against heterophilic interactions during assay development, as well as quality control of the final assay reagents.

These interactions can lead to a signal even in the absence of the analyte, producing false-positive results.

Our Immunoassay Interference Blocker portfolio provides a powerful selection of blocker designed to prevent interferences with conjugated detection reagents. The best blocker for your detection system depends on the particular enzyme or conjugates in use and may include a nonactive mutated enzyme (mutein) or a customized solution. 

Such interactions increase assay background noise and can result from the use of a variety of surface types, including coated/ uncoated microtiter plate wells and other disposables.

Our Bovine Serum Albumin (BSA) blocker solutions are designed to effectively block all types of solid phases involved in immunoassays. These blockers cover a broad spectrum of interactions that might occur in the complex network between assay surfaces, assay components, and target analytes. Efficiently blocking surface interference reduces background signals, increases assay sensitivity and dynamics, and improves reagent stability.