Product Portfolio

Immunoassay Interference Blocker

Optimal solutions for reliable immunoassays


False and unreliable assay outcomes due to interferences can lead to misinterpretation of patient results and inappropriate treatment. In turn, filed complaints lead to mistrust of all products under a brand, and trigger costly and time-consuming troubleshooting and assay rework. Optimal blocking technology can be the critical element that eliminates potential  interferences and positions your product above the competition.

Rely on Roche CustomBiotech for an optimal interference blocking solution that is easily incorporated into your diagnostic immunoassay, and builds efficiencies into your operations. With our Immunoassay Interference Blocker portfolio, it is possible to have it all - efficient blocking performance, consistent quality, a secure supply, and competitive costs.


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Elevate your business with successful immunoassays

Antibody interferences

Heterophilic antibodies and rheumatic factors (RF) can cause significant interferences in any immunoassay. Such antibodies are commonly referred to as Human-Anti-Animal-Antibodies (HAAAs). 

In a patient sample they can interact with analyte-specific antibodies from an assay system. These interactions decrease measurement accuracy and can produce false-positive or false-negative results. Most diagnostic immunoassay reagents use mouse monoclonal antibodies, so human anti-mouse antibodies (HAMA) are the most common cause of interferences.

Up to 10% of test samples contain HAMA antibodies, and their characteristics vary greatly with respect to isotype, specificity, and concentration range. Our immunoassay interference blocker toolbox provides effective and highly specific HAMA interference blocking solutions that account for type of interaction and specific assay design. Plus, our HAMA-interference controls enable evaluation of assay accuracy against heterophilic interactions during assay development, as well as quality control of the final assay reagents.

Detection interferences

Detection interference can arise from heterophilic antibodies in patient samples cross-reacting with assay detection components, such as streptavidin or alkaline phosphatase labels of reagents. 

These interactions can lead to a signal even in the absence of the analyte, producing false-positive results.

Our Immunoassay Interference Blocker portfolio provides a powerful selection of blocker designed to prevent interferences with conjugated detection reagents. The best blocker for your detection system depends on the particular enzyme or conjugates in use and may include a nonactive mutated enzyme (mutein) or a customized solution. 

Surface interferences

Surface interferences arises from nonspecific interactions between serum/plasma proteins or hydrophobic analytes in a test sample and unprotected solid-phase surface materials in the immunoassay. 

Such interactions increase assay background noise and can result from the use of a variety of surface types, including coated/ uncoated microtiter plate wells and other disposables.

Our Bovine Serum Albumin (BSA) blocker solutions are designed to effectively block all types of solid phases involved in immunoassays. These blockers cover a broad spectrum of interactions that might occur in the complex network between assay surfaces, assay components, and target analytes. Efficiently blocking surface interference reduces background signals, increases assay sensitivity and dynamics, and improves reagent stability.

Regulatory disclaimers are listed on the respective product pages.

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