KAPA2G HotStart DNA Polymerase
from Thermus aquaticus, expressed in E. coli
For further processing only.
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Will be supplied as "KAPA2G HotStart DNA Polymerase". Unit of measure is "ml".
KAPA2G HotStart DNA Polymerase material number and pack size: Material Number Pack Size 08918686103 25,000 U - Antibody-mediated hot start 2nd generation mutant of Taq, specifically designed for fast and robust PCR. The product consists of 1 mL of antibody-mediated HotStart DNA polymerase at 25 U/µL.
- Fast and robust DNA amplification of targets with varying GC-content and samples containing PCR inhibitors.
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KAPA2G HotStart DNA Polymerase is a second-generation Taq polymerase engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq. In addition to speed, it provides consistent amplification across a broad range of amplicon types (GC- and AT-rich), eliminating the need for optimization using multiple enzymes and protocols. It enables robust PCR performance, high sensitivity, and improved tolerance to common inhibitors such as NaCl, EtOH, SDS and Urea.
Volume activity: ≥ 25 U/μL
Unspecific endonucleases (plasmid DNA): Not detectable up to 10 U using pBR 322 DNA / 16 hours/ +37°C
Exonucleases: Not detectable up to 10 U using
λ-DNA / 16 hours/+37°C
E. coli DNA: Average Ct value of no template control is ≥ 2.0 cycles later than the average Ct
value of the 100 fg DNA standard