Test kit for the Cedex Bio Analyzer
For use in quality control / manufacturing process only.
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Sucrose Bio material number and pack size: Material Number Pack Size 07705557001 200 tests
- Kit for determination of Sucrose. Kit for determination of sucrose
- Sucrose can be used as source of carbon and energy in cell cultures. Use of sucrose instead of glucose in the culture medium offers the advantage of lower osmolality (based on gramm of carbohydrate). Furthermore, producing therapeutic antibodies, the use of sucrose in the cell culture affects the oligosaccharide maturation on the recombinant proteins, resulting in an increase in the percentage of high mannose N-glycan species, as well as a concomitant reduction in fucosylation, which increases the antibody-dependent cell-mediated cytotoxicity for the antibody. For control of culture media and for monitoring in a running culture process, the sucrose concentration can be determined on a Cedex Bio or Cedex Bio HT Analyzer with high reliability and convenience.
Method: Enzymatic photometric assay using hydrolysis by invertase and phosphorylation of glucose by hexokinase.