ddTTP, Sequencing Grade
sodium salt, 10 mM
For further processing only.
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Will be supplied as "Dideoxy-TTP, Na, Sequencing Grade, Solution". Unit of measure is "μmol".
ddTTP, Sequencing Grade material number and pack size: Material Number Pack Size 12158205103 100 ml 1,000 μmol - For consistent performance in sequencing applications and primer extension reactions, use dideoxyribonucleotides from the leading manufacturer of nucleotides. ddTTP, Sequencing Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The pH is adjusted to match conditions for amplification.
- ddTTP, Sequencing Grade, in dNTP mixes acts as chain terminator in sequencing and primer extension reactions. The high purity and carefully designed manufacturing process are ideally suited for the manufacturing of in vitro diagnostics.
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Nomenclature: 2',3'-Dideoxy-thymidine-5'-triphosphate
Formula: C10H17N2O13P3
Molecular weight: 466.2 D Appearance: Clear, colorless solution
pH value: 8.1-8.5
ddTTP (1 μmol ≙ 8.4 A260 units, pH 7.0): 10.0-11.0 mmol/L
ddTTP (HPLC): ≥98 area%
ddTDP (HPLC): ≤1.5 area%
DNases/RNases: Negative
Nicking activity: Negative
A250/A260: 0.60±0.02
A280/A260: 0.83±0.03
A290/A260: 0.31±0.02
Stability: At -15 to -25°C within specification range for 24 months.