from fish sperm, lyophilizate
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DNA material number and pack size: Material Number Pack Size 10223638103 custom fill
- This DNA preparation can serve for prevention of nonspecific binding in hybridization experiments. DNA is resuspended in sterile double-distilled water at the desired concentration and sheared to an average fragment length of 100 to 3,000 bp.
- Use this preparation of single-stranded genomic DNA fragments to prevent nonspecific binding in membrane or in situ DNA hybridization experiments. It can be added directly to the hybridization mix with no need for prior sonification or denaturation.
The DNA is sonicated. The length of the DNA fragments is mostly in the range of 50 to 600 bp (not verified). UV absorption maximum is at 258 nm.
Appearance: White to slightly grey lyophilizate
DNA content (A260; phosphate buffer, 0.1 mol/L; pH 7.0): ≥15 AB/mg lyophilizate
DNA content (based on Porganic): ≥70%
Porganic (Ptotal-Pi): ≥60 μg/mg lyophilizate
Pi: ≤3 μg/mg lyophilizate
Na (flame photometric): 6±2%
Protein (Lowry): ≤50 μg/mg lyophilizate
Stability: At +2 to +8°C within specification range for 12 months.