Tetramethylrhodamine-5-dUTP
1 mM solution, lithium salt
For further processing only.
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Will be supplied as "Tetramethylrhodamine-5-dUTP". Unit of measure is "nmol".
Tetramethylrhodamine-5-dUTP material number and pack size: Material Number Pack Size 11542907103 custom fill - Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation and in the random-primed labeling reactions. Tetramethylrhodamine-5-dUTP is a substitute for dTTP in nick-translation reactions and in the random-primed labeling technique for DNA labeling, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling. Tetramethylrhodamine-labeled probes show red fluorescence and are suitable for use in in situ hybridization for direct fluorescence detection. Multiple fluorescence labeling using Fluorescein-12-dUTP (yellow fluorescence) or other dye-labeled deoxynucleotides is possible.
- Tetramethylrhodamine-5-dUTP is used for nonradioactive labeling of DNA. This modified nucleotide is a substrate for:
- Terminal Transferase
- DNA polymerase I (holoenzyme and Klenow fragment)
- Taq DNA polymerase
- Reverse transcriptase (e.g., Transcriptor Reverse Transcriptase and other reverse transcriptases)
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Formula: C37H36N5O18P3Li4
Molecular weight: 959.4 D Appearance: Clear, red solution
Tetramethylrhodamine-5-dUTP (1 μmol ≙ 70.0 A551 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test (in situ hybridization): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.