1 mM solution
For further processing only.
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Fluorescein-12-dUTP material number and pack size: Material Number Pack Size 11375601103 custom fill
For other than the listed concentrations please inquire.
- Enzymatic nonradioactive labeling reagent for cDNA synthesis, PCR, random primed labeling, nick-translation or primer extension.
- Use Fluorescein-12-dUTP as a substrate for:
- Terminal Transferase
- DNA polymerase I (holoenzyme and Klenow fragment)
- Taq DNA polymerase
- Reverse transcriptase (e.g., from AMV and M-MuLV)
Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments.
Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.
Molecular weight: 1018.4 D Appearance: Clear, yellow solution
Fluorescein-12-dUTP (1 μmol ≙ 63.3 A495 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC, including isomere): 85.0-100.0 area%
Function test using In Situ Cell Death Detection Kit, Fluorescein (in situ hybridization): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.