Collagenase I/II
lyophilizate, mammalian free
Blended Proteolytic Enzyme for Tissue Dissociation. For Further Processing only.
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Will be supplied as "Collagenase I/II, mammalian free". Unit of Measure is "PC".
Collagenase I/II material number and pack size: Material Number Pack Size 05349907103 mg - The product is intended for enzymatic dissociation of a broad range of tissues, enabling isolation of single cells.The enzymes are manufactured for applications in research where high purity, consistent quality and low levels of bacterial endotoxins are required for reproducible high cell yield and viability. The kit contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other.
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Collagenase I content (target 322 mg) Range/value 258 - 386 mg
Collagenase II content (target 215 mg) Range/value 172 - 258 mg
Collagenase I b,c part of Collagenase I ≤ 10% (area)
HPLC purity: total peak areas (Col I + Col II) ≥ 85%
Stability at -25°C to -15°C: within specificationrange for 24 months.