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cOmplete His-Tag Purification Resin

pre-charged with Ni2+, ready-to-use

For further processing only.

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Order information
cOmplete His-Tag Purification Resin material number and pack size:
Material Number Pack Size
08778850001 800 ml settled resin volume
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Overview
In contrast to other available resins, which are primarily based on NTA or IDA chelator chemistry, this resin tolerates buffers that contain EDTA. Because EDTA is a known inhibitor of metalloproteases - which are frequently present in any cell type - this chromatography material provides the option to protect your target protein by supplementing your buffers with EDTA.

This resin therefore allows the easy purification of your target protein, while using an effective protection against degradation. The His-Tag binding principle allows a standard one-step isolation procedure, generally for all low- and high-molecular weight proteins. Using a special strong-binding Ni2+ chelator, minimal contamination of your fractions with metal ions is ensured, safeguarding your downstream applications. 
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Application
cOmplete His-Tag Purification Resin can be used for small or large-scale purification of His-tagged proteins in biopharma manufacturing, yielding highly purified proteins from crude lysates. This resin can be used for batch or column chromatography procedures, and allows for target protein purification using both native and denaturing conditions. cOmplete His-Tag Purification Resin is compatible with common reducing agents and chelators while preventing contamination of the protein preparation with heavy metals.
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Specification
cOmplete His-Tag Purification Resin is a sepharose-based, pre-charged, ready-to-use Ni2+ chelate resin for small and large-scale purification of His-tagged proteins. It allows for the production of highly pure proteins from crude lysates, using a one-step purification process.
Matrix: Sepharose-CL 6B
Bead size: 45 to 165 μM
Binding Capacity: ≥40 mg protein per mL
Note: The binding capacity of the resin to varíous types of proteins may vary according to the protein characteristics such as the molecular size of the protein.