Cholesterol Oxidase
from Brevibacterium species, expressed in E.coli, lyophilizate
For further processing only.
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Will be supplied as "ChOD, Brevibacterium rec.". Unit of measure is "MU".
Cholesterol Oxidase material number and pack size: Material Number Pack Size 11479709103 custom fill - Oxidoreductase that catalyzes the interconversion of cholesterol to cholest-4-en-3-one.
- Use Cholesterol Oxidase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Esterase, Catalog Nos. 10 129 046 103, 10 262 609 103, 11 015 923 103, 11 520 857 103 or 11 641 735 103.
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Nomenclature: Cholesterol:oxygen oxidoreductase
Molecular weight: 60 kD (native and SDS)
Isoelectric point: ~5.0
Michaelis constant (Phosphate buffer, 0.5 mol/l, pH 7.5; +25°C):
Cholesterol: 1 x 10-4 mol/L
Inhibitors: Hg2+, ZnCl2, SDS
Activators: Non ionic detergents
pH optimum: 5.5-8.0 (see figure)
Temperature dependence: See figure
pH stability: 5.0-10.0 (see figure)
Thermal stability: Up to +55°C (see figure)
Storage and Stability: No decrease in activity over 6 weeks at +35°C (see figure)
Specificity:
cholesterol 100%
pregnenolon 52%
stigmasterol 17%
dehydroandrosterone 0.5%
androsterone 0%
estradiol 0%
cholate 0% Appearance: Yellow lyophilizate
Solubility: Clear, yellowish solution in water (c=10 mg/mL)
pH value: 6.0-7.0
Activity (+25°C, cholesterol): 10-20 U/mg lyophilizate
Protein (Biuret): 10-30 mg/100 mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Oxidase activity):
Catalase: ≤6.0
Glucose oxidase: ≤0.01
"NADH oxidase": ≤0.01
Uricase: ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry. -
