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Cholesterol Esterase, chemically modified

from Pseudomonas species, lyophilizate

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Cholesterol Esterase, chemically modified material number and pack size:
Material Number Pack Size
11641735103 custom fill
Will be supplied as "Cholesterol Esterase Modified". Unit of measure is "MU".
Hydrolase that splits fatty acids from sterols.
Use Cholesterol Esterase, chemically modified in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 11 479 709 103, or 10 634 522 103.
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10-5 mol/L
Inhibitors: Heavy metals such as Cu2+, Ag+, Zn2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure for product Cat. No. 11 520 857 103)
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure for product Cat. No. 11 520 857 103)
Thermal stability: Below +20°C (see figure for product Cat. No. 11 520 857 103)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.
Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/mL)
Activity (+25°C, cholesterol oleate): ≥10 U/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤1.00
Glycerokinase: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.001
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Store dry.