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Cholesterol Esterase, Grade I

from Pseudomonas species, lyophilizate

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
Cholesterol Esterase, Grade I material number and pack size:
Material Number Pack Size
11520857103 custom fill
Will be supplied as "CE, Ps.species, Lyo., SQ". Unit of measure is "MU".
Hydrolase that splits fatty acids from sterols.
Use Cholesterol Esterase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 10 634 522 103, or 11 479 709 103.
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10-5 mol/L
Inhibitors: Heavy metals such as Cu2+, Ag+, Zn2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure)
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure)
Thermal stability: Below +20°C (see figure)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.
Remark: This Cholesterol esterase is especially suited for liquid stable applications with extended shelf life requirements.
Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, cholesterol oleate): ≥100 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Protein (Biuret): No limit
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤1.00
Glycerokinase: ≤0.001
Glucose oxidase: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.001
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Store dry.