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Cholesterol Esterase, Grade II

from Pseudomonas species, lyophilizate

For customers in the European Economic Area: Contains SVHC: octyl/nonylphenol ethoxylates. For further processing on its own or in a mixture as part of an IVD method and under controlled conditions only – acc. to Art. 56 (3) and 3 no. 23 REACH Regulation.

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Order information
Cholesterol Esterase, Grade II material number and pack size:
Material Number Pack Size
11015923103 custom fill
Will be supplied as "Cholesterol Esterase from Pseudom.species". Unit of measure is "MU".
Hydrolase that splits fatty acids from sterols.
Use Cholesterol Esterase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 10 634 522 103 or 11 479 709 103.
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10-5 mol/L
Inhibitors: Heavy metals such as Cu2+, Ag+, Zn2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure for product Cat. No. 11 520 857 103)
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure for product Cat. No. 11 520 857 103)
Thermal stability: Below +20°C (see figure for product Cat. No. 11 520 857 103)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.
Appearance: Slightly yellowish lyophilizate
Solubility: Clear, colorless or yellowish to brownish solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Ficoll (colorimetric): 40-80 mg/100 mg lyophilizate
Activity (+25°C, cholesterol oleate): ≥30 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Protein (Biuret): 0.1-0.4 mg/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤200 U/mg lyophilizate
Glycerokinase: ≤0.001
GOD: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.005
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months.