Uricase
from Arthrobacter protophormiae, lyophilizate
For further processing only.
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Will be supplied as "Uricase from Arthrobact. protophormiae". Unit of measure is "MU".
Uricase material number and pack size: Material Number Pack Size 10828475103 custom fill - Oxidase that catalyzes the oxidation of uric acid to 5-hydroxyisourate which decomposes to allantoin under in vitro conditions.
- Use Uricase in diagnostic tests for the determination of uric acid and for the elimination of uric acid interferences.
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Nomenclature: Urate:oxygen oxidoreductase
Molecular weight: ~170 kD, with four subunits of ~40 kD
Michaelis constant (Phosphate buffer, 0.1 mol/L, pH 8.0; +25°C):
Urate: 6.6 x 10-5 mol/L
Stabilizer/activators: EDTA is good for stabilization. DTT or DTE may show a stabilizing effect depending on reagent composition. Triton X-100 (1-2 mL/L) may show an activating effect.
Inhibitors: Zn2+, Cl- (Tris-HCI buffer is not suitable) and borate inhibit strongly. NaN3, 0.1% does not inhibit.
pH optimum: 9.0 (see figure). Roche uric acid reagent contains phosphate buffer, pH 7.8. For these conditions, high activity and higher stability of the Uricase are achieved.
Temperature dependence: See figure, above +50°C there is a decrease in activity due to lower O2 concentration.
pH stability: 6.5-10.0 (see figure)
Thermal stability: +20 to +60°C (see figure)
Specificity: Uricase is specific for urea. Appearance: White lyophilizate
pH value (c=10 mg/mL in water): 6.7-7.5
Activity (+25°C, urate): ≥20 U/mg lyophilizate
Specific activity: ≥50 U/mg protein
Protein (Biuret): No limit
Contaminant (expressed as percentage of Uricase activity):
Catalase: ≤0.5
Stability: At +2 to +8°C within specification range for 12 months. Store dry. -
